三叶肽因子(TFF1、TFF3)在结肠息肉中的表达及其与临床病理参数的关系  

Expression of trefoil peptide (TFF1,TFF3) in colonic polyps and its relationship with clinicopathological parameters

作　　者：张海玲 马春涛 刘欣 ZHANG Hailing;MA Chuntao;LIU Xin(The First Hospital of Shijiazhuang,Shijiazhuang,Hebei 050000,China;Shijiazhuang Cardio Cerebral Vascular Hospital,Shijiazhuang,Hebei 050000,China)

机构地区：[1]石家庄市第一医院,石家庄050000 [2]石家庄市心脑血管医院,石家庄050000

出　　处：《国际检验医学杂志》2018年第12期1455-1459,共5页International Journal of Laboratory Medicine

基　　金：石家庄市科技局科技支撑计划项目(12146883)

摘　　要：目的评估三叶肽因子(TFF1、TFF3)在结肠息肉组织中的表达情况及其与临床病理参数的关系。方法选择结肠息肉患者120例作为结肠息肉组(包括增生性息肉组40例及腺瘤性息肉组80例),另选择结肠癌患者30例(结肠癌组)及正常结肠黏膜者20例(正常结肠黏膜组)作为对照。采用实时荧光定量PCR、蛋白质印迹及免疫组织化学等方法检测各组织中TFF1和TFF3的表达情况并分析其与临床病理参数的关系。结果 TFF1蛋白在正常结肠黏膜、增生性息肉组织、腺瘤性息肉、结肠癌组织中表达阳性率分别为0、0、53.8%、80.0%,差异均有统计学意义(χ~2=66.614,P<0.05)。TFF3蛋白在正常结肠黏膜、增生性息肉组织、腺瘤性息肉、结肠癌中表达阳性率分别为90.0%、77.5%、55.0%、30.0%,差异有统计学意义(χ~2=24.688,P<0.05)。4组总体TFF1mRNA、TFF3mRNA、TFF1蛋白、TFF3蛋白的表达比较,差异均有统计学意义(P<0.05)。与轻度异型增生性息肉相比,中重度异型增生性息肉中TFF1蛋白表达显著增加(t=2.760,P=0.009),而TFF3蛋白表达显著降低(t=2.556,P=0.015),差异有统计学意义;与管状腺瘤相比,绒毛状腺瘤性息肉中TFF1蛋白表达水平显著增加(t=2.549,P=0.013),而TFF3蛋白表达水平显著降低(t=2.108,P=0.038),差异有统计学意义。结论 TFF1和TFF3的表达情况与结肠恶性癌变过程密切相关,可作为结肠良性疾病鉴别和结肠癌早期诊断的生物标志物。Objective To evaluate the expression of trefoil peptides（TFF1,TFF3）in colonic polyps and its relationship with clinicopathological parameters.Methods 120 cases of colon polyps were selected as the colon polyps group,including 40 cases of hyperplastic polyps and 80 cases of adenomatous polyps,and 30 cases of colon cancer（colon cancer group）and 20 cases of normal colonic mucosa（normal colon mucosa group）were selected as controls.The expression of TFF1 and TFF3 in various tissues were detected by q-RT-PCR,Western Blot and immunohistochemistry,and their relationship with clinicopathological parameters was analyzed.Results The positive rates of TFF1 in normal colon mucosa,hyperplastic polyp tissue,adenomatous polyp and colon cancer were 0,0,53.8% and 80.0%,respectively and the differences were statistically significant（χ~2=66.614,P〈0.05）.The positive rates of TFF3 in normal colon mucosa,hyperplastic polyp tissue,adenomatous polyp and colon cancer were 90.0%,77.5%,55.0%and 30.0%,respectively.and the differences were statistically significant（χ~2=24.688,P〈0.05）.The differences of TFF1 mRNA,TFF3 mRNA,TFF1 protein,TFF3 protein expressions in the four groups were statistically significant.TFF1 protein expression in moderate and severe dysplasia polyp tissues increased significantly compared with those in mild dysplasia polyp tissues（t=2.760,P=0.009）,while the expression of TFF3 significantly decreased（t=2.556,P=0.015）;the expression of TFF1 protein in villous adenomatous polyp tissues increased significantly compared with that in tubular adenoma tissues（t=2.549,P=0.013）,while the expression of TFF3 protein decreased significantly（t=2.108,P=0.038）.Conclusion The expression of TFF1 and TFF3 is closely related to the process of malignant colonic cancinogenesis,and can be used as a biomarker for the differential diagnosis of colon benign diseases and early diagnosis of colon cancer.

关 键 词：三叶肽 结肠息肉 临床病理参数 生物标志物 

分 类 号：R735.2[医药卫生—肿瘤]