TTC染色指数配合Logistic方程鉴定山葡萄种质抗寒性  

Identification of cold resistance in Vitis amurensis germplasms base on TTC staining index and Logistic equation

作　　者：赵滢[1] 艾军[1] 杨义明[1] 王振兴[1] 刘迎雪[1] 何伟[1] 刘海双[1] Zhao Ying;Ai Jun;Yang Yiming;Wang Zhenxing;Liu Yingxue;He Wei;Liu Haishuang(Institute of Special Animal and Plant Sciences,Chinese Academy of Agriculture Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《农业工程学报》2018年第11期174-180,共7页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-29-7);中国农业科学院创新工程协同创新项目(CAAS-XTCX2016017004);中央级公益性科研院所基本科研业务费专项(1610342016011)

摘　　要：为了能简便、准确地鉴定山葡萄种质抗寒性,该研究以40份山葡萄种质1 a生休眠期枝条为试材,利用连有图像分析软件的扫描仪对各低温处理下枝段染色图像进行可视化评估,配合Logistic方程建立回归方程,确定其低温半致死温度。结果表明,枝段染色指数随温度降低而下降,染色指数变化规律符合Logistic方程;40份山葡萄种质低温半致死温度在-39.41～-29.75℃范围内,拟合度在0.956 4～0.999 3范围内,种质抗寒性差异明显;各种质低温半致死温度与其平均隶属度及田间萌芽率均呈极显著负相关,相关系数分别为-0.883 03和-0.876 70。研究结果证明TTC染色图像可视化评估配合Logistic方程鉴定山葡萄种质抗寒性可行,同时为山葡萄种质抗寒性评价及抗寒亲本选择提供了简单、可靠的鉴定方法。Amur grape（Vitis amurensis Rupr.） is the most cold-resistant species in Vitis, and the important parent material in hardiness breeding. For the cold resistance, the dormant shoots have high heritability. However, there might be some differences on cold hardiness among amur grape germplasms in the field. A prerequisite to conducting research in the area of cold hardiness is a reliable method to determine tissue viability. So far, there were no readily available methods to evaluate cold resistance of amur grape shoot, which affected the further excavation and utilization of cold-resistant germplasm. Primarily the triphenyl tetrazolium chloride（TTC） test was used in the identification of cold hardiness for many woody plants as a qualitative response, and if the red formazan derivative was produced, the tissue was considered to be viable, but whether this convenient method was suitable for amur grape or how to use it was not clear. The research was to set up a quick and reliable method for cold-resistant evaluation in V. amurensis germplasms at the dormancy stage. The 40 one-year-old dormant shoots of amur grape germplasms were collected as test materials. Eight freezing temperatures were -15,-20,-25,-30,-35,-40,-45 and -50 ℃, respectively. The chill treatment lasted 12 h and then the germplasms were recovered for 4 h at room temperature after chilling treatment. Both the cooling and heating rates were set 4.0 ℃/h. Then 10 sections with the thickness of 5 cm were cut from the shoots, added with 10 mL 0.5% TTC and dyed for 4 d in 30 ℃ incubator avoiding light. The staining areas of the longitudinal section were projected onto an LA2400 scanner attached to a Win RHIZOTM Image Analysis System（Regent Instruments Inc., Canada）. The level of cold injury of each shoot was recorded as 5 levels based on the area of dyeing in the whole longitudinal section. Then staining index was calculated at related level. The results showed that staining indices and related freezing temperatures presented a typical invert

关 键 词：种质 温度 山葡萄 抗寒性 TTC染色 可视化评估 LOGISTIC方程 

分 类 号：S663.1[农业科学—果树学]