塞来昔布对急性出血坏死性胰腺炎炎症介质的影响  

Study on the effect of Celecoxib on inflammatory mediators of acute hemorrhagic necrotic pancreatitis

作　　者：李明轩 辛辰 王忠琼 LI Mingxuan;XIN Chen;WANG Zhongqiong(l.Clinical Medicine School of Southwest Medical University,Luzhou,Sichuan 646000,Chin;Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China)

机构地区：[1]西南医科大学临床医学院,四川泸州646000 [2]西南医科大学附属医院,四川泸州646000

出　　处：《现代医药卫生》2018年第13期1968-1971,共4页Journal of Modern Medicine & Health

摘　　要：目的探讨塞来昔布(Celecoxib)对急性出血坏死性胰腺炎(AHNP)炎症介质水平的影响。方法将36只SD大鼠随机分为对照组(S组)、AHNP组和Celecoxib预处理组(C+AHNP组),每组12只。采用逆行胰胆管注射法制造大鼠AHNP模型。采集大鼠3、6、12、24 h血液,每个时相点3只,用硝基还原法观察各时相点各组存活大鼠血清一氧化氮(NO)水平的变化,放射免疫法测定肿瘤坏死因子α(TNF-α),动态浊度法定量检测血清内毒素水平。结果 AHNP组大鼠血清NO水平呈进行性上升趋势,其在3、6 h的水平明显低于其余两组,12 h的水平与S组相近,但在24 h的水平明显高于S组;C+AHNP组3,6,12 h的血清NO水平与S组相近,但显著高于AHNP组,第12小时NO水平达高峰,随后明显回落,在24 h略高于S组;AHNP组大鼠血清TNF-α和内毒素水平均显著增高,C+AHNP组大鼠预处理后TNF-α水平显著降低,但内毒素水平无明显影响。结论 Celecoxib具有抑制TNF-α、调节NO等炎症介质的转录与释放,但对急性胰腺炎内毒素水平无影响。Objective To investigate the effect of Celecoxib on the levels of inflammatol7 mediators of acute hemor- rhagic necrotic pancreatitis （AHNP）. Methods 36 SD rats were randomly divided into control group （S group）, AHNP group and Celecoxib pretreatment group （C＋AHNP group）, and each group was 12 rats. AHNP model was builded by retrograde pan-creaticobiliary injection. Collecting rats blood in 3,6,12,24 h, 3 rats at each time. Using the nitro reduction method to observe the level of serum NO at each time point of each group survival, TNF-α was measured by radioimmunoassay, dynamic turbidi-metric method was used to detect the level of serum endotoxin quantitatively. Results The serum NO level of AHNP group showed a progressive increase, the levels of 3,6 h were significantly lower than those of S group, the level of 12 h was similar to S group, but the level of 24 h was significantly higher than that of S group. C＋AHNP group significantly improved serum NO levels of 3,6,12 h in AHNP rats, and the NO level reached the peak in 12 h, then obviously decreased, at 24 h slightly higher than that of S group. The levels of TNF-α and endotoxin in rats of AHNP group were significantly increased. Celecoxib pretreatment significantly decreased the levels of TNF-α, but has no effect on the level of endotoxin. Conclusion Celecoxib can inhibit the transcription and release of TNF-α and other ilfflammatory mediators such as NO, but it has no effect on endotoxin level in acute pancreatitis.

关 键 词：吡唑类 胰腺炎 急性坏死性 动物 实验 塞来昔布 炎症介质 

分 类 号：R576[医药卫生—消化系统]