芒果乙烯受体ETR1和ERS1基因的克隆及序列分析  

Cloning and sequence analysis of ethylene receptor ETR1 and ERS1 genes from mango(Mangnifera indica L.)

作　　者：李丽[1,2] 孙健 易萍[1] 饶川艳 李昌宝 何雪梅 零东宁[1] 肖占仕 盛金凤[1] 唐雅园[1] 李杰民 郑凤锦[1] LILi;SUNJian;YIPing;RAOChuan-yan;LIChang-bao;HEXue-mei;LINGDong-ning;XIAOZhan-shi;SHENGJin-feng;TANGYa-yuan;LIJie-min;ZHENGFeng-jin(Agro-foodScienceandTechnologyResearchInstitute,GuangxiAcademyof AgriculturalSciences,Nanning 530007Chin;GuangxiCropGeneticImprovementLaboratory,Nanning 530007,China)

机构地区：[1]广西农业科学院农产品加工研究所,南宁530007 [2]广西作物遗传改良重点开放实验室,南宁530007

出　　处：《南方农业学报》2018年第6期1053-1060,共8页Journal of Southern Agriculture

基　　金：广西自然科学基金项目(2015GXNSFBA139102);广西创新驱动发展专项项目(桂科AA17204038);广西农业科学院基本科研业务专项项目(桂农科2015YT86;桂农科2018YM04)

摘　　要：【目的】克隆芒果乙烯受体ETR1和ERS1基因(Mi ETR1和Mi ERS1),并进行生物信息学分析,为研究乙烯受体在芒果成熟和衰老过程中的调控机制提供理论参考。【方法】以芒果为材料,应用RACE技术扩增Mi ETR1和Mi ERS1基因的c DNA序列,并应用生物信息学方法对其编码的氨基酸序列进行预测分析。【结果】克隆获得的Mi ETR1基因c DNA序列全长2570 bp,开放阅读框(ORF)2220 bp,编码739个氨基酸;Mi ERS1基因的c DNA序列全长2171 bp,ORF1890 bp,编码629个氨基酸;Gen Bank登录号分别为KY002681和KY002682。Mi ETR1和Mi ERS1蛋白均为含跨膜结构的非分泌型疏水蛋白,属于乙烯受体ETR1家族成员,以Ser为主、Thr为辅的磷酸化修饰调控乙烯信号转导,均含有3个典型的模块,即GAF结构域、His KA结构域和HATPase_c结构域,Mi ERS1蛋白较Mi ETR1缺少REC结构域。不同物种ETR1和ERS1蛋白的氨基酸序列具有高度保守性,Mi ETR1和Mi ERS1均与甜橙(Citrus sinensis)和克莱门柚(Citrus clementina)的ETR1和ERS1蛋白亲缘关系较近,但二者分别聚在两个不同的分支上,表明两个蛋白存在一定的遗传分化。【结论】乙烯受体基因在芒果成熟和衰老过程中发挥调控作用。【Objective】Ethylene receptor ETR1 and ERS1 genes（MiETR1 and MiERS1）from mango（Mangnifera indica L.）were cloned,and bioinformatics analysis was conducted in order to provide theoretical reference for further study of functional mechanism of the ethylene receptors in mango ripening and senescencing.【Method】Using mango fruit as material,full-length cDNA sequences of MiETR1 and MiERS1 by RACE technology was amplified,and the encoded amino acid sequences were predicted by bioinformatics method.【Result】The full-length cDNA sequence of cloned gene Mi-ETR1 was 2570 bp,an open reading frame（ORF）of 2220 bp,encoding 739 amino acids. The full-length cDNA sequence of cloned gene MiERS1 was 2171 bp,and it was consisted of an ORF of 1890 bp,encoding 629 amino acids. The Gen-Bank accession numbers for the two were KY002681 and KY002682. Bioinformatic analysis showed that the MiETR1 and MiERS1 proteins were non secreted hydrophobic protein with transmembrane domain,belonging to the ETR1 family member of ethylene receptor with Ser-based,Thr-assisted phosphorylation-modulatory. MiETR1 and MiERS1 proteins contained three typical modules,namely GAF domain,HisKA domain and HATPase_c domain. MiERS1 protein lacked REC domain compared with MiETR1. The amino acid sequences of ETR1 and ERS1 proteins in different species were highly conserved. MiETR1 and MiERS1 had high homologous with the ETR1 and ERS1 proteins of Citrus sinensis and Citrus clementina. However,the two clustered on two different branches,indicating that there was genetic differentiation between the two proteins at certain extents.【Conclusion】Ethylene receptor gene plays a regulatory role in mango fruit maturation and senescence.

关 键 词：芒果 乙烯受体 基因克隆 序列分析 

分 类 号：S667.703.6[农业科学—果树学]