酶催化功能混杂性研究进展  

A Brief Overview of Catalytic Promiscuity of Enzymes

作　　者：杨键[1] 龙丽娟[1] YANG Jian;LONG Lijuan(CAS Key Laboratory of Tropical Marine Bio Resources and Ecology,Guangdong Key Labora tory of Marine Materia Medica,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,Guangdong,510301,China)

机构地区：[1]中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室广东省海洋药物重点实验室,广东广州510301

出　　处：《广西科学》2018年第3期253-257,共5页Guangxi Sciences

基　　金：中科院战略性先导科技专项(XDA10030404;XDA13020301);国家自然科学基金青年基金项目(41406193);广东海洋经济创新发展区域示范项目(GD2012-D01-002)资助

摘　　要：酶催化生物体内化学反应,是生命代谢形成运转的动力。与传统观点认为酶具有专一性催化功能相对,近年来生物信息与实验分析都证实了酶具有多种混杂催化功能是普遍存在的现象。在过去的几十亿年里,古老酶一直不断演化以适应变化的环境,形成现代功能多样的酶蛋白家族。基于独特底物结合模式和动态蛋白结构的催化功能混杂性是酶蛋白适应性演化的基础。酶的混杂活性有望被开发应用于药物酶法合成及环境修复领域。本文就酶催化功能混杂性的普遍性、分子机理、可进化性等方面的相关研究进展进行综述。Enzymes, which catalyze chemical reactions in vivo, are considered as the driving forces for the formation of life metabolism. Compared with the traditional view that enzymes have specific catalytic functions,bioinformatics and experimental analysis in recent years have confirmed that enzymes having a variety of hybrid catalytic functions are universal phenomena. Over the past several billion years, ancient enzymes have evolved to adapt to changing environ ments, forming a modern and diverse family of enzyme proteins. Catalytic functional promiscui ty based on unique substrate binding patterns and dynamic protein structure is believed to be the basis for the adaptive evolution of enzyme proteins. The enzymatic miscibility of the enzyme is expected to be exploited in the fields of drug enzymatic synthesis and environmental remedia tion fields. In this paper, we summarized the related research progresses on the universality, molecular mechanism,and evolvability of enzyme catalyzed functional promiscuity.

关 键 词：酶 混杂性 分子进化 环境适应 蛋白动态性 

分 类 号：Q518[生物学—生物化学]