陕西省韩城市利什曼原虫SSU rRNA基因系统发育研究  

Phylogenetic analysis based on small subunit ribosomal RNA(SSU rRNA) of Leishmaniain Hancheng City,Shaanxi Province,China

作　　者：张铮[1] 李欣妍 刘东立[1] 王凤萍 王安礼[1] 王天海 ZHANG Zheng;LI Xin yan;LIU Dong-li;WANG Feng-ping;WANG An-li;WANG Tian-hai(Shaanxi Provincial Center for Disease Control and Prevention,Xi＇an 710054;Health Science Center Xi＇an Jiaotong University,Xi＇an 710061,China;Hancheng City Center for Disease Control and Prevention,Hancheng 715400,China)

机构地区：[1]陕西省疾病预防控制中心,西安710054 [2]西安交通大学医学部,西安710061 [3]陕西省韩城市疾病预防控制中心,西安715400

出　　处：《中国人兽共患病学报》2018年第6期550-554,共5页Chinese Journal of Zoonoses

基　　金：陕西省科技资源共享平台项目-公共卫生检测监测服务平台(2016FWPT-12)

摘　　要：目的了解陕西省韩城市黑热病区利什曼原虫种类及分子遗传特征。方法对来自韩城市的利什曼原虫阳性标本,进行SSU rRNA基因序列扩增,扩增产物进行测序,与其它不同种类、不同地区利什曼原虫的SSU rRNA基因序列进行比对,分析突变位点规律,建立系统发育树。结果有3份标本SSU rRNA基因序列扩增测序成功,其基因序列与杜氏利什曼原虫、夏科氏利什曼原虫、婴儿利什曼原虫的SSU rRNA序列同源性较高,与婴儿利什曼原虫在UQ-II突变区有一个位点的变异,并与新疆荒漠虫株、甘肃省利什曼虫株聚为一类。结论结合流行病学与SSU rRNA序列分析结果,陕西省韩城市黑热病病原体应为婴儿利什曼原虫。We identified the species and molecular genetic characteristics of Leishmania protozoa in Hancheng City,Shaanxi Province.The SSU rRNA genes of Leishmania positive samples were amplified and sequenced.The sequencing results were compared with the SSU rRNA gene sequences of Leishmania in different regions and species reported in the literature.The mutation sites were analyzed and the phylogenetic tree was constructed.Three specimens＇ SSU rRNA gene amplification sequences,which were highly homologous to L.donovani,L.Chagas and L.infantum,were successfully sequenced.The sequences had one base variation,comparing with the L.infantum in the UQ-II mutant region.Phylogenetic tree analysis showed that Leishmaniafrom Shaanxi Province,Gansu Province and L.infantumstrains from Xinjiang were gathered into one cluster.Epidemiological data and SSU rRNA sequence analysis showed that the pathogens of visceral leishmaniasis in Hancheng City,Shaanxi Province should be Leishmania infantum.

关 键 词：利什曼原虫 SSU RRNA 系统发育 分子鉴定 陕西 

分 类 号：R382.2[医药卫生—医学寄生虫学]