纳米二氧化钛与脂多糖对小鼠肝脏抗氧化性能的影响  

Effects of titanium dioxide nanoparticles and lipopolysaccharide on antioxidant function of liver tissues in mice

作　　者：段淑敏 张永亮[1] 王云[1] DUAN Shu-min,ZHANG Yong-liang,WANG Yun(Department of Occupational and Environmental Health Sciences,Peking University of School of Public 100191,Chin)

机构地区：[1]北京大学公共卫生学院劳动卫生与环境卫生学系,北京100191

出　　处：《北京大学学报（医学版）》2018年第3期395-400,共6页Journal of Peking University:Health Sciences

基　　金：北京市自然科学基金(7172116);国家自然科学基金(31400863)资助~~

摘　　要：目的:比较慢性经口暴露不同尺寸二氧化钛(titanium dioxide,Ti O2)对小鼠肝脏组织抗氧化性能的影响,并探讨纳米Ti O2在小鼠肝脏中对脂多糖(lipopolysaccharide,LPS)易感性的影响。方法:将90只4周龄清洁级雄性ICR小鼠分为18组,分别饲喂维持饲料、1%(质量分数)纳米Ti O2(34 nm)饲料、1%(质量分数)亚微米Ti O2(125nm)饲料1个月、3个月、6个月,饲养结束后的第2天再分别灌胃给予0或10 mg/kg LPS,4 h后处死小鼠,记录体重及肝重,并计算肝脏系数,取肝组织制备匀浆测定抗氧化指标,包括总抗氧化能力(total antioxidant capacity,TAOC)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSHPX)、丙二醛(malondialdehyde,MDA)。结果:小鼠的体重改变仅在染毒1个月的情况下出现,表现为各处理组的体重与对照组相比显著下降,肝脏系数在各组均未发生明显变化。染毒1个月、6个月小鼠纳米Ti O2+LPS处理组、亚微米Ti O2+LPS处理组与对照组、纳米Ti O2处理组、亚微米Ti O2处理组和LPS处理组相比,肝脏T-AOC、T-SOD、MDA三项指标有不同程度的升高。染毒3个月小鼠不同尺寸Ti O2处理组的肝脏MDA活性降低。染毒1个月与6个月小鼠不同尺寸Ti O2处理组下各项指标结果间差异无统计学意义,与对照组间差异也无统计学意义。结论:长期口服纳米Ti O2和亚微米Ti O2对成长期小鼠肝脏更容易产生损伤,且损伤既可能是还原损伤也可能是氧化损伤,小尺寸Ti O2会提高小鼠肝脏对LPS的易感性,并随着暴露时间的增加而提高。Objective： To compare the effects of different sized titanium dioxide（ titanium dioxide,Ti O2） on the antioxidant function of liver tissues in mice,and study the effect of Ti O2 nanoparticles on the susceptibility of lipopolysaccharide（ LPS） on liver tissues. Methods： Ninety 4-week-old clean-grade male ICR mice were divided into 18 groups,in which the mice were fed for different feed involving ordinary feed,nanometer Ti O2 feed which meant the feed including 1%（ mass fraction） Ti O2 nanoparticles,and submicron Ti O2 feed which meant the feed including 1%（ mass fraction） Ti O2 submicron particles.Respectively,they were fed for 1 month,3 months and 6 months. On the second day after the feeding,respectively,0 and 10 mg/kg LPS were given by gavage. The mice were harvested after 4 h and the body weight and liver weight for calculating the liver coefficient were recorded. Then the liver tissue homogenates were prepared for determining the antioxidant indexes including the total antioxidant capacity（ TAOC）,total superoxide dismutase（ T-SOD）,glutathione peroxidase（ GSH-PX）,and malondialdehyde（ MDA）. Results： The change of body weight in mice was only discovered in group fed for 1 month,which showed significant decrease of body weight in treatment groups compared with control group. And there was no significant change of the liver coefficient in each group. Compared with control groups,nanometer Ti O2 groups and submicron Ti O2 group,the activity of T-AOC,T-SOD and MDA of nanometer Ti O2＋ LPS group and submicron Ti O2＋ LPS group in which the mice were fed for 1 month and 6 months increased in different degree. And another result was also existing. The MDA activity of liver in different sized treatment groups fed for 3 months decreased. Neither significant difference between the results of different sized Ti O2 treatment groups,nor significant difference among different sized Ti O2 groups and the control groups were observed. Conclusion： Long-term peroral Ti O2 nanoparticles and

关 键 词：二氧化钛 纳米颗粒 抗氧化剂 肝 

分 类 号：R155.3[医药卫生—营养与食品卫生学]