FOXK1通过上皮间充质转化促进胃癌浸润转移研究  

FOXK1 promoting infiltration and metastasis of gastric cancer through epithelial-mesenchymal transition

作　　者：卢洪胜 於樱枝 牟樱红 潘黎明 曹学全 包卫光 章辉 LU Hongsheng

机构地区：[1]台州市中心医院(台州学院附属医院),台州318000 [2]玉环市人民医院

出　　处：《现代实用医学》2018年第4期436-438,564,共4页Modern Practical Medicine

基　　金：浙江省医药卫生科技计划(2018ky909);玉环市科技计划(201741)

摘　　要：目的观察FOXK1在胃癌中的表达,探讨其在胃癌发生发展中的关系及临床意义。方法应用组织芯片及免疫组化En Vision法检测FOXK1、E-cadherin、Vimentin在88例胃癌及正常胃黏膜中的表达。结果 88例胃癌与正常胃黏膜中,FOXK1的阳性表达率分别为96.59%和7.95%,差异有统计学意义(<0.01);FOXK1蛋白表达与胃癌分化程度、T分期、N分期及TNM分期有关(均P<0.01),而与患者性别、年龄及肿瘤大小无关(均P>0.05);FOXK1蛋白表达与E-cadherin表达呈负相关(r=-0.21,P<0.05),与Vimentin表明呈正相关(r=0.63,P<0.05)。结论 FOXK1蛋白异常表达与胃癌的发生发展有关,可作为判断生物学特征标记物。Objective To observe the expression of FOXK1 in gastric cancer and explore its relationship with the occurrence and development of gastric cancer and its clinical significance. Methods The expressions of FOXK1, E-cadherin and Vimentin in 88 cases of gastric cancer and normal gastric mucosa were detected by tissue chip and immunohistochemical En Vision method. Results The positive expression rates of FOXK1 in 88 cases of gastric cancer and normal gastric mucosa were 96.59% and 7.95% respectively, and there was significant difference between them（P 〈0.01）.The expression of FOXK1 protein was associated with T stage, N stage, and TNM stage（all P〈0.01）, but not associated with gender, age, and tumor size（all P〉0.05）. The expression of FOXK1 protein was negatively correlated with E-cadherin expression（r=-0.21, P〈0.05）, and positively correlated with Vimentin expression（r=0.63, P〈0.05）.Conclusion The abnormal expression of FOXK1 protein is related to the occurrence and development of gastric cancer. It can be used as a marker for judging biological marker.

关 键 词：胃癌 FOXK1 EMT 组织芯片 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]