Na_2SeO_3和ZnSO_4对大球盖菇富集液体培养的影响  

Effects of Na_2SeO_3 and ZnSO_4 on Liquid Fermentation of Strophariarugoso-annulata Mycelia

作　　者：曾健勇[1] 赵博[1] 毕文玉 刘春延[1] 张国财[1] ZENG Jianyong;ZHAO Bo;BI Wenyu;LIU Chunyan;ZHANG Guocai(School of Forestry,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《吉林农业大学学报》2018年第2期171-177,共7页Journal of Jilin Agricultural University

基　　金：中央高校基本科研业务费专项(2572017AA18);中央财政林业科技推广示范资金项目[JLT(2016)13]

摘　　要：为开发同时富含人体必需微量元素硒和锌的新膳食补充剂,在实验室现有大球盖菇菌丝液体培养基(土豆200 g/L,葡萄糖20 g/L,酵母浸粉4 g/L,磷酸二氢钾2 g/L,硫酸镁1 g/L,维生素B10.05 g/L)基础上,添加不同浓度的Na_2SeO_3和ZnSO_4对大球盖菇菌丝进行富硒、富锌及硒锌同富液体培养,测定了富集液体培养的大球盖菇菌丝干重、多糖产量、可溶性蛋白产量和有机硒锌产量,探究了Na_2SeO_3和ZnSO_4对大球盖菇富集液体培养的影响。试验结果表明:大球盖菇菌丝硒锌同富液体培养的最佳硒锌浓度为Na_2SeO_320 mg/L、ZnSO_450 mg/L。在此条件下,大球盖菇菌丝干重比对照高96.12%,有机硒产量比对照高237.29倍,有机锌产量比对照高4.22倍,多糖产量比对照高63.64%,可溶性蛋白比对照高78.16%,Na_2SeO_3利用率比Na_2SeO_3浓度为20 mg/L富硒液体培养处理组高30.12%,ZnSO_4利用率比ZnSO_4浓度为50 mg/L富锌液体培养处理组高0.47%。这说明适宜浓度的硒、锌同富液体培养可以显著提高大球盖菇菌丝产量、菌丝活性物质产量和生物转化率。The objective of the present work was to obtain Se-Zn-enriched mycelia of Strophariarugo- so-annulata by optimizing the liquid fermentation medium composition. Orthogonal experiment was designed to optimize the liquid fermentation medium, which is composed of 200 g/L potato, 20 g/L glucose, 4 g/L yeast extract, 2 g/L KH2P04, 1 g/L MgS04 and 0.05 g/L vitamin B1 and is en- riched with Se and Zn by the addition of sodium selenite and zinc sulfate. Dry weight, nutrient yield and bioaccumulation of S. rugoso-annulata mycelia were evaluated. Results show that the optimal concentrations of sodium selenite and zinc sulfate in the Se-Zn-enriched liquid fermentation of S. rugoso-annulata were 20 mg/L and 50 mg/L, respectively. Under the optimal concentration, dry weight and yield of bioactive substances of S. rugoso-annulata were significantly increased by Se-Zn- enriched liquid fermentation. Dry weight was 96.12% higher than control, organic Se yield was 237.29 times higher than control, organic Zn yield was 4.22 times higher than control, polysaccha- rides yield was 63.64% higher than control, soluble proteins yield was 78. 16% higher than eontrol, utilization ratio of NazSeO3was 30. 12% higher than 20 mg/L treatment group of Se-enriched fermen- tation, and utilization ratio of ZnSO4 was 0. 47% higher than 50 mg/L treatment group of Zn-en- riched fermentation.The results suggest that the optimized liquid fermentation medium could be ap- plied to obtain a new dietary supplement enriched in Se and Zn.

关 键 词：大球盖菇 硒 锌 液体培养 有机硒产量 有机锌产量 

分 类 号：S646[农业科学—蔬菜学] TS209[农业科学—园艺学]