一株好氧反硝化细菌的筛选及对土壤脱氮效果研究  

Screening of an Aerobic Denitrification Strain and Its Nitrogen Removal Characteristics for Soil

作　　者：陈寒玉 靳慧征 靳慧霞[3] Chen Hanyu;Jin Huizheng;Jin Huixia(School of Chemistry,Chemical and Environment Engineering,Henan University of Technolog;Henan Technical College of Constructio;Ningbo Institute of Technology,Zhejiang University,Ningbo 315100)

机构地区：[1]河南工业大学化学化工与环境学院,郑州450052 [2]郑州建筑职业技术学院工程管理系,郑州450000 [3]浙江大学宁波理工学院,浙江宁波315100

出　　处：《中国农学通报》2018年第20期55-62,共8页Chinese Agricultural Science Bulletin

基　　金：浙江省宁波市农业攻关项目"设施栽培菜地次生盐渍化修复与控制关键技术研究"(2014C10014);浙江省宁波市科技富民项目"基于无线通信技术和优化模型的菜地智能灌溉系统"(2016C10057);河南工业大学博士科研基金"蜂窝状石墨碳材料的制备与应用"(31400347)

摘　　要：为了探究反硝化生物菌剂于盐渍化土壤修复的应用机制和条件,以好氧反硝化能力为主要指标,以宁波慈溪地区的大棚种植土壤为样本,通过筛选比较进行菌株分离,得到一株高效好氧反硝化菌DPT001。通过形态、生理生化特征和16SDNA基因序列分析对菌种进行了菌属鉴定,并研究了该菌株适宜的生长条件。筛分出的好氧反硝化菌为革兰氏阳性短杆菌,属于克伯氏菌属的Castellaniella脱氮杆菌。在好氧环境下,52 h时NO3-降解率达到92%。菌株的适宜生长条件为中性或弱碱性土壤,盆栽试验表明,以此菌剂制备的生物菌肥(培养的菌种和腐熟花生壳混合)对土壤总盐度和硝酸盐浓度都有明显的降低作用。本研究从大棚土中筛选出的好氧反硝化菌,具有较高的反硝化特性,并优于市售的商品脱氮生物菌剂。To explore the application mechanism and condition of denitrifying biological agent in saline soilremediation, aerobic denitrification capacity was used as the main index, the planting soil of greenhouse inNingbo Cixi was used as the material, the bacterial strain was isolated by screening and comparing. An aerobicbacterial strain with the highest activities was obtained and named DPT001. The strain was identifiedaccording to its morphological, physiological and biochemical properties and 16 S DNA gene sequence analysis.The suitable culture condition of this strain was studied. The results showed that DPT001 was a gram-positivebacillus and was identified as Castellaniella ginsengisoli. In the anaerobic environment, NO3-degradation ratewas 92% in 52 h. The suitable growth conditions were neutral or weak alkaline soil. The pot experimentshowed that the biological bacterial fertilizer（mixed with the strain and decomposed peanut shell） obviouslydecreased the total salt concentration and NO3-concentration. An aerobic denitrifying strain was isolated fromgreenhouse soil and it exhibited high denitrification characteristics and was better than the commercialdenitrification biological bacterial agent.

关 键 词：土壤次生盐渍化 好氧反硝化 菌落特征 生物菌肥 土壤生物修复 

分 类 号：X172[环境科学与工程—环境科学]