人前列腺癌组织拓扑异构酶Ⅱα蛋白的表达水平及其对生化复发的预测意义  

The expression of topoisomerase IIα in prostate cancer tissue and its significance in prediction of prostate cancer recurrence

作　　者：牛吉瑞[1] 王海 唐蕊[1] 孙士恒[1] 姜晓龙[3] 于茵[1] Niu Jirui;Wang Hai;Tang Rui;Sun Shiheng;Jiang Xiaolong;Yu Yin(Department of Urology,Heilongjiang Province Hospital,Haerbin,Heilongjiang Province 150036,China;Department of Urology,Peking Union Medcial College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College;Department of UItrasound,Heilongjiang Province Hospital,Haerbin)

机构地区：[1]黑龙江省医院泌尿外科,哈尔滨150036 [2]中国医学科学院、北京协和医学院北京协和医院泌尿外科 [3]黑龙江省医院超声科

出　　处：《中国男科学杂志》2018年第3期9-11,共3页Chinese Journal of Andrology

基　　金：黑龙江省卫生厅科研课题(2013369)项目资助;黑龙江省卫生计生委科研课题(2017-481)资助

摘　　要：目的研究拓扑异构酶Ⅱα在人前列腺癌组织中的表达水平,探讨其与生化复发的关系。方法回顾性分析我院30例人前列腺癌手术病理切片,其中16例Gleason评分≥7分,14例<7分。所有标本进行拓扑异构酶Ⅱa蛋白表达的免疫组织化学染色。随访患者,应用spss软件进行Kaplan-meirer生化复发的预后分析。结果拓扑异构酶Ⅱα蛋白在确诊为前列腺癌组织中26例呈阳性表达,阳性率为86.7%。拓扑异构酶Ⅱα的表达水平与Gleason评分存在一定相关性。当Gleason评分大于等于7分时,拓扑异构酶Ⅱα阳性表达水平较高;而Gleason评分小于7分时,拓扑异构酶Ⅱα阳性表达水平较低。两组间构成比存在显著性差异(P<0.05)。拓扑异构酶Ⅱα蛋白的表达水平与生化复发率存在一定相关性。拓扑异构酶Ⅱα在生化复发组的表达水平较高,其在未复发组的表达水平较低。结论人前列腺癌组织Gleason评分高,拓扑异构酶Ⅱα蛋白的表达水平高,生化复发率亦高,患者预后差。拓扑异构酶Ⅱα蛋白表达水平可以作为判断前列腺癌患者预后的生化指标。Objective To investigate the expression of topoisomerase Ⅱα in prostate cancer tissue and explore its significance in prediction of prostate cancer recurrence. Methods Thirty prostate cancer specimens including 16 specimens whose Gleason score was more than 7 and 14 specimens whose Gleason score was less than 7, were retrospectively analyzed in our hospital. The expressions of topoisomerase Ⅱα in all the samples were detected by immunohistochemistry. All patients were followed up and the relationship between topoisomerase Ⅱα expression and recurrence free survival was analyzed by Kaplan-meirer. Results 86.7% of prostate cancer tissues had topoisomerase Ⅱα positive response. Gleason scores were correlated with topoisomerase Ⅱα level. When Gleason scores were over 7, the expression level of topoisomerase Ⅱα was high. When Gleason scores were below 7, the expression level was low.The expression level of topoisomerase Ⅱα in the biochemical recurrence free survival group are higher than that in the other group. Conclusion Topoisomerase Ⅱα can help us to confirm the diagnosis of prostate cancer. Topoisomerase Ⅱα expression was found to increase with the known prognostic marker Gleason score.

关 键 词：前列腺肿瘤 肿瘤标记 生物学 DNA拓扑异构酶类 Ⅱ型 

分 类 号：R737.25[医药卫生—肿瘤]