解淀粉芽孢杆菌对免疫抑制肉鸡生长性能和肝脏抗氧化功能的影响  

Effects of Bacillus amyloliquefaciens on growth performance and liver antioxidant function of immunosuppressed broilers

作　　者：应志雄[1] 李悦[1] 张昊[1] 苏伟鹏[1] 黄强[1] 张莉莉[1] 陆兆新[2] 王恬[1] YING Zhi-xiong;LI Yue;ZHANG Hao;SU Wei-peng;HUANG Qiang;ZHANG Li-li;LU Zhao-xin;WANG Tian(College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,Chinas;College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]南京农业大学食品科技学院,江苏南京210095

出　　处：《中国兽医学报》2018年第8期1609-1615,共7页Chinese Journal of Veterinary Science

基　　金：“十二五”国家科技支撑计划资助项目(2013BAD10B02-03)

摘　　要：旨在研究解淀粉芽孢杆菌（Bacillusam yloliquefaciens，BA）对环磷酰胺（cyclophosphamide，CY）介导的免疫抑制肉鸡生长性能和肝脏抗氧化功能的影响。144只1日龄AA雄性肉仔鸡被随机分为3组，即对照组（CON），环磷酰胺处理组（cY）和环磷酰胺-解淀粉芽孢杆菌组（cY-BA），每组6个重复，每个重复8只鸡。CON组和CY组饲喂基础日粮，CY-BA组饲喂添加1．08×10^10CFU／kgBA的试验日粮。于16，17和18日龄对CY组与CY-BA组肉鸡肌肉注射80mg／kg的CY，CON组肌肉注射等量生理盐水，试验期为21d。结果显示，与CON组相比，cY刺激显著降低了CY组肉鸡1～21日龄体增质量、肝脏指数、肝脏总抗氧化能力（total antioxidative capacity，T-AOC）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力和谷胱甘肽还原酶（glutathione reductase，GR）活力（P〈0．05），显著升高了肝脏丙二醛（malondialdehyde，MDA）含量（P〈0．05）；Real-timePCR检测也发现，CY处理显著降低了CY组肉鸡肝脏核转录相关因子2（NF-E2-relatedfactor2，nrf2）和谷胱甘肽过氧化物酶1（glutathione peroxi-dase1，gpxl）的mRNA表达水平（P〈0．05）。与cY组相比，日粮添加BA显著提高了cY-BA组肉鸡21日龄时肝脏T-AOC水平和GSH-Px活力，降低了肝脏MDA水平（P〈0．05）。上述指标在CON与CY-BA组间并无明显差异（P〉0．05）。另外，CY-BA组肝脏GSH含量较CON或CY组均显著升高（P〈0．05）。然而，日粮添加BA并未显著影响CY刺激肉鸡体增质量、肝脏指数、肝脏nrf2和gpxlmRNA水平（P〉0．05）。综合结果表明，CY诱导的免疫抑制损伤了肉鸡生长和肝脏的抗氧化功能，并引起了肉鸡肝脏脂质过氧化程度升高。日粮添加BA具有改善CY介导的免疫抑制肉鸡肝脏抗氧化功能和缓解肝脏氧化损伤的作用。This study was conducted to investigate the effects of Bacillus amyloliquefaciens （BA） on growth performance and liver antioxidant function of immunosuppressed broilers induced by cyclophosphamide （CY）. 144 1-day-old male Arbor Acre broilers were randomly distributed into three groups （CON,CY and CY-BA groups）. Each group consisted of six replicates with 8 broilers per replicate. The broilers of CON and CY groups were fed the basal diets and the broilers of CY- BA group were fed the basal diet supplemented with 1.08x 10^10CFU/kg BA. At 16,17 and 18 d of age,the broilers in CY and CY-BA groups were intramuscularly injected with CY at the dosage of 80 mg/kg, and the broilers in CON group were intramuscularly injected with equivalent saline. Broilers were sacrificed on day 21. Compared with the CON group,CY group showed decreases inbody weight gain,liver index and the activities of hepatic glutathione peroxidase （GSH-Px） and glutathione reductase （GR）, but an increase in the concentration of hepatic malondialdehyde （MDA） （P〉0.05）. Real-time PCR analysis showed that mRNA levels of hepatic NF-E2-related factor 2 （nrf2） and glutathione peroxidase 1 （gpxl） were lower in the CY group than that in the CON group （P〉0.05）. Compared with the CY group,dietary BA supplementation significantly increased GSH-Px activity whereas decreased MDA content in the liver of CY challenged broilers （P〈0.05）. No obvious differences were found in the above parameters between CON and CY-BA groups （P〈0.05）. The content of GSH was markedly increased in the liver of CY-BA group when compared with either CON or CY group,respectively （P〉0.05）. Besides,there were no significant differences in body weight gain,liver index, and levels of nrf2 and gpxl mRNA between CY-BA and CY groups （P〉0.05）. These data suggested that immunosuppression induced by CY inhibited growth,impaired hepatic antioxidant function and resulted in lipid oxidative damage in the liver of broilers. BA supplementat

关 键 词：解淀粉芽孢杆菌 免疫抑制 环磷酰胺 肉鸡 生长性能 抗氧化功能 

分 类 号：S831.5[农业科学—畜牧学] S816.7[农业科学—畜牧兽医]