枳实制白术的炮制工艺优选及HPLC特征图谱分析  

Optimization of Processing Technology of Atractylodis Macrocephalae Rhizoma Processed with Aurantii Fructus Immaturus and Analysis of Its HPLC Fingerprint

作　　者：王芳[1] 于欢[1] 李风琴[1] 张帅杰 刘怀伟 代良敏 李艳[1] 杨明[1] WANG Fang;YU Huan;LI Feng-qin;ZHANG Shuai-jie;LIU Huai-wei;DAI Liang-min;LI Yan;YANG Ming(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Chengdu Baicao Heft Technology Co.Ltd.,Chengdu 611137,China;The Affiliated Hospital of Southwest Medical University,Luzhou 646000,China)

机构地区：[1]江西中医药大学,南昌330004 [2]成都百草和济科技有限公司,成都611137 [3]西南医科大学附属医院,四川泸州646000

出　　处：《中国实验方剂学杂志》2018年第17期6-13,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家级中药炮制技术传承基地项目(国中医药科技[2015]86号);江西中医药大学重点学科青年教师培养计划项目(2015jzzdxk011);江西中医药大学博士启动基金项目(2015BS002);江西省教育厅科学技术研究项目(GJJ170752)

摘　　要：目的：探讨枳实制白术的最佳炮制工艺，建立其HPLC特征图谱。方法：采用“脾虚泄泻”免疫力低下动物模型和大鼠饮水量来评价枳实制白术的健脾祛燥作用；通过对比不同配比枳实制白术饮片的健脾祛燥作用，筛选枳实与白术的最佳配比；采用正交试验，以辛弗林和白术内酯Ⅲ的含量为评价指标，优选枳实制白术的炮制工艺，并建立其特征图谱。结果：10％枳实制白术组的效果最佳。枳实制白术饮片炮制过程中，影响辛弗林及白术内酯Ⅲ的主次因素依次为烘制温度〉炒制温度〉炒制时间，最佳炮制工艺为取生白术饮片100g，加入枳实汁50mL（生药质量浓度0．2g·mL-1），浸泡24h，不时翻动，直至汁尽药透，于110℃炒制6min，取出，于40℃烘箱干燥。枳实制白术有5个共有峰，均能达到较好分离，该分析方法具有良好的精密度、重复性和稳定性，符合特征图谱相关要求。结论：药汁制是中药炮制的重要组成部分，枳实制白术的健脾祛燥作用充分体现了这一炮制特色——增效，纠正偏性。建立的IqPLC特征图谱方法准确、可靠，为枳实制白术的质量控制提供了参考。Objective： To optimize processing technology of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus and establish its HPLC fingerprint. Method： The invigorating spleen and dispelling dryness of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus was investigated by model rats with splenasthenic diarrhea, and then the best ratio of Atractylodis Macrocephalae Rhizoma and Aurantii Fructus Immaturus was screened. Taking overall desirability of contents of synephrine and atractylenolide III as index, orthogonal test was employed to optimize processing technology, and HPLC fingerprint of this processed product was established. Result： The best ratio of Atractylodis Macrocephalae Rhizoma toAurantii Fructus Immaturus was 9 ： 1. The factors influenced contents of synephrine and atractylenolide HI were followed by baking temperature 〉 frying temperature 〉 frying time, optimum processing technology of this processed product was as follows： added Aurantii Fructus Immaturus juice 50 mL （crude drug concentration of 0.2 g -mL-1） into 100 g of Atractylodis Macrocephalae Rhizoma pieces, soaked 24 hours, fried 6 min at 110 ~（2 , dried at 40 ~C in a oven. Five common peaks were selected in Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus. Among the obtained fingerprint, all of the detected peaks were separated effectively. The accuracy, repeatability and stability of this method were satisfied. Conclusion： The invigorating spleen and dispelling dryness of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus can reflect the processing characteristics of increasing efficiency and correcting bias. The established HPLC fingerprint can provide reference for the quality control of this processed product.

关 键 词：枳实 白术 白术内酯Ⅲ 辛弗林 药汁制 健脾祛燥 

分 类 号：R22[医药卫生—中医基础理论] R283[医药卫生—中医学]