结肠癌细胞裂解物致敏的树突状细胞-细胞因子诱导杀伤细胞抗自体结肠癌细胞效应研究  

Tumor lysate-pulsed dendritic cell with cytokine-induced killer cells improve antitumor effect on autologous colon cancer cells

作　　者：朱克祥[1,2,3] 王锦明[2] 袁得峰 张正聪[2,3] 吕鹏飞[2,3] 白小平 李汛[1,2,3] 潘藤 ZHU Ke-xiang;WANG Jin-ming;YUAN De-feng;ZHANG Zheng-eong;LYU Peng-fei;BAI Xiao-ping;LI Xun;PAN Teng(Department of General Surgery,The First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一医院普外三科,甘肃兰州730000 [2]兰州大学第一医院东岗院区普外科,甘肃兰州730000 [3]兰州大学第一医院甘肃省生物治疗与再生医学重点实验室,甘肃兰州730000 [4]沈阳军区总医院肝胆外科,辽宁沈阳110016

出　　处：《临床军医杂志》2018年第8期878-881,共4页Clinical Journal of Medical Officers

基　　金：甘肃省卫生行业计划项目(GSWSKY-2015-51)

摘　　要：目的探讨负载结肠癌细胞裂解产物的树突状细胞(DC)-细胞因子诱导的杀伤细胞(CIK)对自体结肠癌细胞的体外杀伤作用。方法选取结肠癌患者切除的肿瘤组织反复冻融,制备结肠癌细胞裂解产物,将结肠癌细胞裂解产物与自体DC共培养。将负载自体结肠癌细胞裂解产物致敏的DC与CIK共培养,形成抗原(Ag)-DC-CIK细胞,检测Ag-DC-CIK细胞的免疫活性以及对自体结肠癌细胞的杀伤效应。结果 Ag-DC-CIK组和DC-CIK组CD3+、CD56+双阳性细胞比例分别为(31.0%±4.1%)和(29.0%±3.4%),显著高于CIK组的(19.0%±2.5%),组间比较,差异有统计学意义(P<0.05)。在同等效靶比条件下,Ag-DC-CIK组对自体肿瘤细胞的杀伤能力强于DC-CIK组和CIK组,组间比较,差异有统计学意义(P<0.05)。结论 Ag-DC-CIK细胞对自体结肠癌细胞的杀伤效应显著强于DC-CIK和CIK细胞。Objective To evaluate the activity that tumor lysate-pulsed dendritic cell（ DC） co-cultured with cytokine-induced killer（ CIK） cells in vitro to suppress and kill autologous colon cancer cells. Methods The tumor tissue resected by colon cancer patients was repeatedly freeze-thawed and thawed. The sensitized DC and CIK cells loaded with the cleft product of autogenous colon cancer cells were co-cultured to form Ag-DC-CIK cells,and the immunological activity of Ag-DC-CIK cells and the killing effect on autogenous colon cancer cells were detected. Results The positive CIK cells CD3 ＋ and CD56 ＋ in the Ag-DC-CIK group and the DC-CIK group were（ 31. 0% ± 4. 1%） and（ 29. 0% ± 3. 4%）,which were significantly higher than（ 19. 0% ± 2. 5%） in the CIK group,and the difference between the groups was statistically significant（ P〈0. 05）. Under the condition of the same equivalent target ratio,the killing ability of autologous colon cancer cells in the Ag-DC-CIK group was better than that of the DC-CIK group and the CIK group,and the difference was statistically significant（ P〈0. 05）. Conclusion Ag-DC-CIK cells are more efficacious than DC-CIK cells and CIK cells on the effects and activity to kill autologous colon cancer cells in vitro.

关 键 词：结肠癌 树突状细胞 细胞因子 杀伤细胞 细胞免疫治疗 

分 类 号：R735.3[医药卫生—肿瘤]