原发性肝细胞肝癌YAP、FOXP1 mRNA的表达及对患者预后的影响  

Expression of YAP and FOXP1 mRNA in hepatocellular carcinoma and its influence on prognosis of patients with hepatocellular carcinoma

作　　者：陈橼[1] 周元[1] 尹必辉 李怀亮 侯卫晓 周益龙[1] 邵冰峰[1] 张素青[1] 徐爱兵[1] 张一心[1] CHEN Yuan;ZHOU Yuan;YIN Bi-hui;LI Huai-liang;HOU Wei-xiao;ZHOU Yi-long;SHAO Bing-feng;ZHANG Su-qing;XU Ai-bing;ZHANG Yi-xin(Department of Surgery,Nantong Tumour Hospital,Nantong University,Nantong 226300,Jiangsu,China;Department of Clinical Medicine,Graduate School,Nantong University School of Medicine,Nantong 226019,Jiangsu,China)

机构地区：[1]南通大学附属肿瘤医院外科,南通226300 [2]南通大学医学院研究生院临床医学系,南通226019

出　　处：《中国临床医学》2018年第4期549-554,共6页Chinese Journal of Clinical Medicine

基　　金：南通市指令性科技计划(MS22015010);南通市指导性科技计划(HS149069;HS149073);南通市卫计委青年基金(WQ2015047);江苏省南通市卫计委重点病种的临床规范化诊疗项目(MS32015028);江苏省南通市卫计委医学重点学科建设项目(YY201207)~~

摘　　要：目的:探讨肝细胞肝癌(hepatocellular carcinoma,HCC)中Yes-associated protein(YAP)、Forkhead-box P1(FOXP1)mRNA的表达及其对患者无病生存期(disease-free survival,DFS)的影响。方法:采用实时定量反转录聚合酶链反应(real-time quantitative reverse transcriptase polymerase chain reaction,real time RT-PCR)检测114例HCC患者石蜡组织中YAP、FOXP1mRNA表达水平。采用回归分析研究YAP、FOXP1 mRNA表达水平对HCC患者DFS的影响。结果:YAP、FOXP1mRNA表达水平与HCC肿瘤直径、TNM分期、AFP水平正相关(P<0.05)。单因素分析发现患者DFS与肿瘤分化程度、TNM分期、肝内肿瘤个数、AFP水平、HBV感染、YAP及FOXP1高表达有关;进一步多因素分析发现患者肝癌术后复发与患者HBV感染和YAP、FOXP1高表达有关,YAP、FOXP1高表达影响HCC患者预后(P<0.05)。结论:FOXP1、YAP高表达影响HCC患者DFS预后。Objective：To investigate the expression of Yes-associated protein （YAP） and Forkhead-box P1 （FOXP1） mRNA in hepatocellular carcinoma （HCC） and its influence on patients’ disease-free survival （DFS）. Methods：Real-time quantitative reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect the mRNA expression of YAP, FOXP1 mRNA in paraffin embedded tissues of 114 HCC patients. Regression analysis was performed to estimate the effect of YAP and FOXP1 mRNA expression levels on the DFS time of HCC patients. Results：The mRNA expression levels of YAP and FOXP1 were significantly associated with tumor diameter, TNM stage, and AFP （P〈0.05）. The expression level of YAP was directly correlated with that of FOXP1 （r=0.76, P〈0.000 1）. A univariate analysis showed that tumor differentiation, TNM stage, gross classification, α fetoprotein, HBV infection, YAP and FOXP1 expression were predictors of DFS in HCC patients. Furthermore, multivariate Cox proportional hazards regression analysis indicated that HBV infection and YAP/FOXP1 co-expression were independent prognostic factors for HCC （P〈0.05）. Conclusions：High expression of FOXP1 and YAP mRNA in HCC tissue might affect the DFS prognosis of patients with HCC.

关 键 词：Yes相关蛋白 叉头框P1 肝细胞肝癌 

分 类 号：R735.7[医药卫生—肿瘤]