晚期糖基化终末产物受体促进子宫内膜癌裸鼠皮下移植瘤的生长  

Receptor for advanced glycation endproducts promote the growth of endometrial carcinoma of transplantation tumor in nude mice

作　　者：郑璐[1] 孟斐[1] 李彤[1] 刘伟[1] Zheng Lu;Meng Fei;Li Tong;Liu Wei(Department of Gynecology and Obstetrics,Central Hospital of Shenyang Medical College,Liaoning Shenyang 110024,China.)

机构地区：[1]沈阳医学院附属中心医院妇产科,辽宁沈阳110024

出　　处：《现代肿瘤医学》2018年第17期2656-2659,共4页Journal of Modern Oncology

基　　金：辽宁省自然科学基金项目(编号:2014010136-301);沈阳医学院科技基金项目(编号:20131021);沈阳医学院博士科研启动基金(编号:20186067)

摘　　要：目的:探讨晚期糖基化终末产物受体(RAGE)是否能促进子宫内膜癌裸鼠皮下移植瘤的生长。方法:利用腺病毒介导的RNAi干扰技术下调HEC-1A细胞中RAGE的表达,将干扰前后的HEC-1A细胞接种到裸鼠皮下构建移植瘤模型,观察RAGE对裸鼠皮下移植的子宫内膜癌细胞生长的影响。结果:RAGE sh RNAi腺病毒干扰的HEC-1A细胞不再表达RAGE蛋白,干扰后的HEC-1A细胞形成的移植瘤体积及质量显著小于干扰前细胞形成的肿瘤(P<0.01)。结论:RAGE促进子宫内膜癌裸鼠皮下移植瘤的生长。Objective： To explore whether the receptor for advanced glycation endproducts（ RAGE） promotes the growth of endometrial carcinoma of transplantation tumor in nude mouse. Methods： Construction of HEC-1 A cell lines with knockdown of RAGE by RAGE sh RNAi adenovirus transfection of HEC-1 A cells. HEC-1 A cells or RAGE sh RNAi-transfected HEC-1 A cells were subcutaneously injected into the right armpit of female BALB/c nude mice,the long and short diameters of tumor were recorded in every 5 days. At 20 d after tumor transplantation,the animals were sacrificed to remove tumor. The maximum and minimum diameters of tumor were measured,the tumor volume was calculated,and the tumor was weighed and recorded. Results： RAGE expression was not detected in RAGE sh RNAi adenovirus-transfected HEC-1 A cells. The volume and mass of transplantation tumor in the transfected group were both significantly lower than those in the non-transfected group（ P 〈0. 01）. Conclusion： Receptor for advanced glycation endproducts promote the growth of endometrial carcinoma of transplantation tumor in nude mice.

关 键 词：子宫内膜癌 晚期糖基化终末产物受体 裸鼠 移植瘤 

分 类 号：R737.33[医药卫生—肿瘤]