HA14-1联合顺铂诱导人小细胞肺癌细胞凋亡的研究  

Experiment of HA14-1 combined cisplatin in treating human small cell lung cancer

作　　者：张强[1] 边明艳 谭明旗[1] Zhang Qiang;Bian Mingyan;Tan Mingqi(Department of the Second Respiratory,Shengjing Hospital,China Medical University,Liaoning Shenyang 110000,China;Jinqiu Hospital of Liaoning Province,Liaoning Shenyang 110016,China.)

机构地区：[1]中国医科大学附属盛京医院第二呼吸内科,辽宁沈阳110000 [2]辽宁省金秋医院,辽宁沈阳110016

出　　处：《现代肿瘤医学》2018年第17期2677-2682,共6页Journal of Modern Oncology

摘　　要：目的:观察HA14-1及其联合顺铂对人小细胞肺癌NCI-H446细胞凋亡诱导作用并探讨其可能的作用机制,探讨其对Bcl-2、Bax表达的的影响。方法:选用人小细胞肺癌NCI-H446细胞株为研究对象,分为空白组、DDP组、HA14-1组及DDP+HA14-1组,分别作用24、48 h后,采用MTT法检测细胞抑制率,流式细胞仪检测细胞凋亡,Real time PCR法比较细胞内Bcl-2、Bax表达变化。结果:随着HA14-1及DDP浓度的增加,细胞抑制率逐渐增加,细胞凋亡率增高,联合用药组较单独用药组细胞的抑制率,凋亡率均增高,HA14-1作用细胞后,Bcl-2表达水平下降,Bax表达水平增高。结论:HA14-1可以诱导人小细胞肺癌凋亡,并增加肺癌细胞对DDP的化疗作用。Objective： To observe HA14-1 and HA14-1 combined cisplatin induced human small cell lung cancer NCI-H446 cell to apoptosis and explored its possible mechanism,affected the expression of Bcl-2,Bax.Methods： The human small cell lung cancer NCI-H446 cell line was the object of this study,which was divided into blank control group,DDP group,HA14-1 group and DDP ＋ HA14-1 group,the role of 24 h,48 h,respectively,then cell inhibition rate were determined by MTT,the apoptotic rate of cells were measured by flow cytometry,the expression of intracellular Bcl-2,Bax gene were compared by Real time PCR. Results： With the increased concentrations of HA14-1 and DDP,cell inhibition rate increased gradually,the apoptotic rate was increased,the rate of cell inhibition and apoptosis of combination therapy group were higher than in the same dose group alone,HA14-1 roled in cells,Bcl-2 expression was lower,Bax expression was increased. Conclusion： HA14-1 could induce human small cell lung cancer apoptosis and increased the lung cancer cells to chemotherapy the role of DDP.

关 键 词：HA14-1 NCI-H446 小细胞肺癌 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]