PD-1、CTLA-4、BLTA在食管鳞状细胞癌患者外周血中的表达及临床意义  

Expression of PD-1,CTLA-4 and BLTA in the peripheral blood of patients with esophageal squamous cell carcinoma and its clinical implications

作　　者：郝贺[1] 李幸[1] 吴玲彦 郭晓金[1] 曹静[1] 杨春旺 张晓 汪治宇[1] HAO He;LI Xing;WU Lingyan;GUO Xiaojin;CAO Jing;YANG Chunwang;ZHANG Xiao;WANG Zhiyu(Department of Immuno-Oncology,Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Department of Cardiology Physician,Second Hospital of Shijiazhuang)

机构地区：[1]河北医科大学第四医院肿瘤科(免疫),石家庄050011 [2]石家庄市第二医院心内科

出　　处：《实用肿瘤学杂志》2018年第4期303-308,共6页Practical Oncology Journal

摘　　要：目的检测食管鳞癌患者外周血中抑制性协同刺激因子受体PD-1、CTLA-4、BLTA表达情况,并分析其临床意义。方法选取2016年6月—2017年4月河北医科大学第四医院胸外科90例食管鳞状细胞癌患者(其中50例患者行手术治疗)和40例健康对照者为研究对象,收集其外周血液标本,采用酶联免疫吸附方法检测血清中可溶性PD-1(s PD-1)、可溶性CTLA-4(s CTLA-4)及可溶性BLTA(s BLTA)的表达水平。结果食管鳞癌组血清中s PD-1、s CTLA-4及s BLTA水平均明显高于对照组(P<0.05);食管鳞癌组手术前后血清中s PD-1、s CTLA-4及s BLTA水平均无统计学差异(P>0.05)。s PD-1和s BLTA的表达水平与临床病理特征无相关性,s CTLA-4的表达水平与TNM分期相关(P<0.05),与T分期、N分期、肿瘤体积大小、肿瘤部位、组织分化程度、性别、年龄无相关性;血清中s PD-1、s CTLA-4及s BLTA两两间无相关性(P>0.05)。结论食管鳞癌患者血清中s CTLA-4较正常人表达升高,且s CTLA-4的表达水平与TNM分期相关,说明血清中s CTLA-4表达水平与病情发展变化有一定相关性。Objective The aim of this study was to evaluate the expression of programmed cell death 1（ PD-1）,cytotoxic T lymphocyte associated antigen-4（ CTLA-4） and B and T lymphocyte attenuator（ BTLA） in the peripheral blood of patients with esophageal squamous cell carcinoma（ ESCC） and their clinical significance was analyzed. Methods A total of 90 patients with esophageal squamous cell carcinoma and 40 healthy controls at the department of Thoracic Surgery of the Fourth Hospital of Hebei Medical University were enrolled and collected their peripherals from June 2016 to April 2017. Blood samples from 50 patients underwent surgery from 90 patients and 40 healthy controls were assayed by enzyme-linked immunosorbent assay for soluble PD-1（ s PD-1）,soluble CTLA-4（ s CTLA-4） and soluble BTLA（ s BTLA）. Results The serum levels of s PD-1,s CTLA-4 and s BLTA in the esophageal squamous cell carcinoma group were significantly higher than those in the normal control group（ P 0. 05）; the serum levels of s PD-1,s CTLA-4 ands BLTA had no differences between before and after surgery（ P 0. 05）. There was no correlation between the level of s PD-1 and s BLTA and clinicopathological features. The expression level of CTLA-4 was related to the TNM stage（ P 0. 05）,but there were no correlation with T stage,lymph node status,tumor size,tumor location,tumor cell differentiation,sex and age. There was also no correlation amongst s PD-1,s CTLA-4 and s BLTA expression. Conclusion The serum level of s CTLA-4 in patients with esophageal squamous cell carcinoma is higher than that in normal individuals,and the expression level of s CTLA-4 is correlated with the TNM stage,suggesting that the expression level of s CTLA-4 in serum has a certain correlation with the development of esophageal squamous cell carcinoma.

关 键 词：食管鳞状细胞癌 程序性死亡分子1 细胞毒性T淋巴细胞相关抗原 B和T淋巴细胞衰减蛋白 

分 类 号：R735.1[医药卫生—肿瘤]