miR-let-7b通过调控相关细胞周期蛋白影响皮肤黑色素瘤的增殖和凋亡  

mi R-let-7b Affects the Proliferation and Apoptosis of Cutaneous Melanoma by Regulating the Relevant Cyclin

作　　者：秦琴[1] 宁花兰 陈小燕[1] 陈少英[1] 张艳珍[1] 陈大江 QIN Qin,NING Hua-lan,CHEN Xiao-yan,CHEN Shao-ying,ZHANG Yan-zhen,CHEN Da-jiang(Department of Dermatology,Shenzhen Bao＇an People＇s Hospital, Shenzhen 518101,Guangdong, Chin)

机构地区：[1]深圳市宝安区人民医院皮肤科,广东深圳518101

出　　处：《中国美容医学》2018年第7期79-82,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨mi R-let-7b通过调控相关细胞周期蛋白影响皮肤黑色素瘤的增殖和凋亡。方法:q PCR法检测mi R-let-7b在黑色素瘤组织和细胞株中的表达情况;双荧光素酶报告基因检测mi R-let-7b与CCND1之间的相互作用;MTT增殖实验检测抑制mi R-let-7b后黑色素瘤细胞的增殖能力的变化情况;流式细胞术检测抑制mi R-let-7b后黑色素瘤细胞的凋亡行为的变化情况。结果:与癌旁正常皮肤组织相比,黑色素瘤组织中mi R-let-7b的表达水平相对上调,与其他黑色素瘤细胞株相比,A375细胞中mi R-let-7b表达最高;双荧光素酶实验证实mi R-let-7b能与CCND1的3’UTR特异性结合,可以调控CCND1的表达与活性;抑制mi R-let-7b的表达后可以抑制黑色素瘤细胞的增殖能力;同时可以促进黑色素瘤细胞的凋亡行为。结论:mi R-let-7b可以调控CCND1的表达从而影响黑色素瘤细胞的增殖和凋亡。Objective To investigate the effect of mi R-let-7 b on the proliferation and apoptosis of melanoma by regulating related cell cycle proteins. Methods Expression of mi R-let-7 b in melanoma tissues and cell lines was detected by q PCR. Double luciferase reporter gene was used to detect the interaction between mi R-let-7 b and CCND1. MTT proliferation assay was used to detect the proliferation of melanoma cells after inhibition of mi R-let-7 b. Flow cytometry was used to detect the apoptotic behavior of melanoma cells after inhibition of mi R-let-7 b. Results Compared with adjacent normal skin tissue, the expression level of mi R-let-7 b in melanoma tissue was relatively up-regulated. Compared with other melanoma cell lines, the expression of mi R-let-7 b was the highest in A375 cells. Double luciferase assay confirmed that mi R-let-7 b could specifically bind to the 3＇ UTR of CCND1 and regulate the expression and activity of CCND1. mi R-let-7 b can inhibit the proliferation of melanoma cells, And can promote the apoptosis of melanoma cells. Conclusion mi R-let-7 b can regulate the expression of CCND1 and affect the proliferation and apoptosis of melanoma cells.

关 键 词：mi R-let-7b 黑色素瘤 CCND1 凋亡 增殖 

分 类 号：R739.5[医药卫生—肿瘤]