梅花鹿骨提取物中2个新多肽的分离与鉴定  

Two new polypeptides from extract of deer bone extract

作　　者：任易[1] 张云封[1] 刘文静[1] 赵云芳[1] 宋月林 何利群 夏瑞雪 屠鹏飞[1] 李军[1] REN Yi;ZHANG Yun-feng;LIU Wen-jing;ZHAO Yun-fang;SONG Yue-lin;HE Li-qun;XIA Rui-xue;TU Peng-fei;LI Jun(Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;Harbin Gloria Pharmaceuticals Co.,Ltd.,Harbin 150025,China)

机构地区：[1]北京中医药大学中药学院中药现代研究中心,北京100029 [2]哈尔滨誉衡制药有限公司,黑龙江哈尔滨150025

出　　处：《中国中药杂志》2018年第12期2557-2562,共6页China Journal of Chinese Materia Medica

基　　金：教育部新世纪优秀人才支持计划项目(NCET-13-0693);国家“重大新药创制”科技重大专项(2013ZX09402201001)

摘　　要：利用C4300,Sephadex G-50和半制备HPLC等色谱技术对鹿瓜多肽注射液组方成分梅花鹿骨提取物的多肽类化学成分进行研究,分离纯化得到2个多肽类化合物,其精确相对分子质量分别为m/z 1 489.868 5和m/z 2 113.870 6。通过MALDI-TOF-MS及ExplorerTM软件从头测序（De Novo测序）分析,鉴定了2个多肽类化合物的N端（氮端）一级序列,分别为NH2-Gly-Pro-Val-Gly-Pro-Thr-Gly-Pro-Val-Gly-Ala-Ala-Gly-Pro-Se-Gly-Pro-Asp（梅花鹿骨18肽,1）和NH2-Ala-Gly-Pro-Ala-GlyPro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Pro-Asp-Ser-Try-Asp（梅花鹿骨23肽,2）。1和2均为新多肽。Two new polypeptides were isolated and purified from the extract of deer bone（ constitutive part of Cucumis and Cervus polypeptide injection） by various column chromatography including C4300 and Sephadex G-50,as well as semipreparative HPLC.Their N-terminal amino acid sequences were identified by De Novo sequencing on the basis of MALDI-TOF-MS data and ExplorerTM software. The N-terminal amino acid sequences of polypeptides were identified as NH2-Gly-Pro-Val-Gly-Pro-Thr-Gly-Pro-Val-Gly-AlaAla-Gly-Pro-Ser-Gly-Pro-Asp（ Mei18 peptide,1） and NH2-Ala-Gly-Pro-Ala-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-Gly-Pro-Leu-GlyPro-Pro-Asp-Ser-Try-Asp（ Mei23 peptide,2）,respectively. Mei18 and Mei 23 peptides are new polypeptides.

关 键 词：鹿瓜多肽注射液 鹿骨 多肽 MALDI-TOF-MS 化学成分 

分 类 号：R284[医药卫生—中药学]