响应面法优化精制沙丁鱼油EPA和DHA富集工艺  

Optimization of EPA and DHA enrichment process of refined sardine oil by response surface methodology

作　　者：张蒙娜 宋恭帅 彭茜 薛静 朱凤仙 戴志远 ZHANG Mengna;SONG Gongshuai;PENG Xi;XUE Jing;ZHU Fengxian;DAI Zhiyuan(Institute of Seafood,Zhejiang Gongshang University,Hangzhou 310012,China;Kagawa University,Takamatsu 7610795,Japan;State Key Laboratory of Aquatic Products Processing of Zhejiang Province,Hangzhou 310012,China)

机构地区：[1]浙江工商大学海洋食品研究院,杭州310012 [2]香川大学,日本高松7610795 [3]折江省水产品加工技术研究联合重点实验室,杭州310012

出　　处：《中国油脂》2018年第8期42-46,共5页China Oils and Fats

基　　金：科技部国际科技合作项目(2014DFA32880);浙江省重大科技专项重点项目(2012C03009-4)

摘　　要：以乙酯型精制沙丁鱼油(EPA含量(15.41±0.13)%,DHA含量(8.00±0.03)%)为原料,在尿酯比、包合温度、醇尿比、包合时间4个单因素试验的基础上,采用响应面分析法优化尿素包合法富集工艺。结果表明:最优工艺条件为尿酯比3.5∶1、包合温度0℃、醇尿比2.6∶1、包合时间1.2h,此条件下,产物中EPA+DHA含量为(78.12±0.12)%(EPA含量(53.35±0.10)%;DHA含量(24.77±0.02)%),与理论值78.17%基本一致。Using refined sardine oil ethyl ester（EPA content（ 15.41 - 0.13 ）%, DHA content（8.00 - 0. 03 ）% ）as raw material, the effects of urea - ester ratio, inclusion temperature, ethanol - urea ratio and inclusion time on the EPA and DHA enrichment process were studied by single factor experiment, and the response surface methodology was used to optimize the urea inclusion enrichment process. The re- sults showed that the optimal process conditions were obtained as follows ： urea - ester ratio 3.5： 1, inclu- sion temperature 0℃, ethanol - urea ratio 2.6： 1, and inclusion time 1.2 h. Under these conditions, the total content of EPA and DHA was（78.12-0.12）% （EPA content（53.35 ±0.10）%, DHA content （24.77±0.02）% ）, which was consistent with the theoretical value of 78.17%.

关 键 词：精制沙丁鱼油 二十碳五烯酸(EPA) 二十二碳六烯酸(DHA) 响应面法 乙酯型 

分 类 号：TS225.2[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ641[轻工技术与工程—食品科学与工程]