蒙古马长期高负荷运动训练前后肌肉发育关键基因的表达研究  

The Investigation to Expression of Key Genes for Muscle Development before and after Long-term High Loading Exercise in Mongolian Horses

作　　者：白东义[1] 苏日嘎[1] 芒来[1] 乌伊罕 赵一萍[1] 李蓓[1] 格日乐其木格 张心壮 陶克涛[1] 赵若阳 图挌琴 青柏 旭仁其木格 BAI Dong-yi;Suriga;DUGARJAVIIN Mang-lai;Wuyihan;ZHAO Yi-ping;LI Bei;BOU Gerelchimeg;ZHANG Xin-zhuang;TAO Ke-tao;ZHAO Ruo-yang;Tugeqin;QING Bai;Xurenqimuge(Equine Research Center,Inner Mongolia Key Laboratory of Equine Genetics,Breeding and Reproduction,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Center for Disease Comprehensive Control and Prevention,Hohhot 010030,China)

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古自治区马属动物遗传育种与繁殖重点实验室,内蒙古农业大学马属动物研究中心,呼和浩特010018 [2]内蒙古自治区综合疾病预防控制中心,呼和浩特010030

出　　处：《畜牧兽医学报》2018年第8期1617-1624,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31472070;31360538); 内蒙古自治区自然科学基金项目(2015BS0318); 呼和浩特市科技计划项目(2014-农-16); 内蒙古自治区科学技术厅项目(201603008)

摘　　要：旨在比较研究长期高负荷运动训练前后蒙古马肌肉组织中与肌肉发育相关关键基因的表达量,从而为马匹耐力训练方案的制定提供数据支持。本研究以3匹5岁雌性蒙古马为试验对象,对其进行为期4个月的连续性高负荷耐力运动训练,并在实施运动训练前后采集臀中肌肌肉用于后续研究。采用RT-qPCR和Western blot方法,比较分析长期高负荷运动训练前后蒙古马肌肉组织中MYL-2和TNNC1基因在转录和翻译水平的表达量。结果表明,经长期高负荷运动训练后蒙古马骨骼肌中MYL-2和TNNC1基因在mRNA水平的表达量极显著高于运动训练前(P<0.01)。在蛋白水平,MYL-2的表达量在运动训练后有了极显著的增加(P<0.01),而TNNC1在运动后虽有增加但未达到显著水平(P>0.05)。长期的高负荷运动训练对蒙古马肌肉发育相关基因MYL-2和TNNC1的表达均有促进作用,从而能够促进肌肉的发育,对蒙古马的耐力形成至关重要。The aims of this study were to compare expression quantity of key genes for muscle development before and after long-term high loading exercise in Mongolian horse,and to provide data support for the development of horse endurance training programs.Three female Mongolian horses of 5-years-old were used and continuous high loading endurance exercise were carried out for continue 4 months,and the gluteus medius were collected before and after exercise,respectively.RT-qPCR and Western blot were used to analyze the expression quantity of MYL-2 andTNNC1 genes at transcription and translation levels before and after long-term high loading exercise in Mongolian horses.The results showed that the expression quantity of MYL-2 and TNNC1 genes in Mongolian horses muscle after exercise were significantly higher than that before exercise at mRNA level（P〈0.01）.The expression quantity of MYL-2 significantly increased after exercise（P〈0.01）,the expression quantity of TNNC1 increased after exercise at protein level（P〈0.05）.The long-term high loading exercise promote the expression of MYL-2 and TNNC1 genes related to Mongolian horses muscle development.It is important to form endurance of Mongolian horse.

关 键 词：蒙古马 长期高负荷运动训练 转录 翻译 基因差异表达 

分 类 号：S821.2[农业科学—畜牧学]