猪源奇异变形杆菌的分离鉴定与耐药性分析  

Isolation,identification and drug-resistance analysis of Proteus mirabilis from swine

作　　者：董萌萌 徐彦召[1] 杭柏林[1] 杨磊 孙亚伟[1] 王青[1] 胡建和[1] DONG Meng-meng;XU Yan-zhao;HANG Bo-lin;YANG Lei;SUN Ya-wei;WANG Qing;HU Jian-he(College of Animal Science and Veterinary Medicine,Henan Institute of Science and Technology,Xinxiang 453003,Chin)

机构地区：[1]河南科技学院动物科技学院,河南新乡453003

出　　处：《中国兽医杂志》2018年第5期82-85,I0008,共5页Chinese Journal of Veterinary Medicine

基　　金：河南省高校科技创新团队支持计划(15IRTSTHN);河南科技学院2015年大学生"百农英才"创新项目(BNYC2015-2-09)

摘　　要：为了对河南省某猪场疑似细菌感染病例进行确诊,本试验采集了病猪肠道中的样品从中分离到1株革兰阴性短杆菌,进行了培养特性观察、生化试验、药敏试验以及16S rRNA基因序列分析。结果显示,分离菌的生化特性、培养特性基本符合奇异变形杆菌的特征,16S rRNA基因序列与奇异变形杆菌的同源性在98%以上,将该分离菌株命名为ZB17;序列分析表明,该分离菌株与猪源奇异变形杆菌(HQ259935.1和KR133627.1)的同源性最高,与大肠杆菌、沙门菌,克雷伯菌的同源性相对较低;分离株具有较强的致病性和多重耐药性,对复达欣、庆大霉素、奈替米星、阿米卡星敏感;但对氨苄西林、美洛西林、先锋霉素IV、先锋霉素V、先锋霉素VI、卡那霉素、新霉素、四环素、强力霉素耐药。本试验为猪源奇异变形杆菌的分离、鉴定及治疗提供了参考。In order to diagnose the suspected bacterial infection in a pig farm in Henan province,we collected the samples of the sick pig＇s intestines and isolated 1 strain of gram negative bacillus. The cultural characteristics,biochemical properties test,drug sensitivity test and 16 S rRNA gene sequence analysis were carried out. Results showed that the biochemical characteristics of the isolated bacteria were in accordance with Proteus mirabilis. The homology of 16 S rRNA gene sequence with Proteus mirabilis was above 98%.The isolated strain was designated as ZB17. Sequence analysis showed that the isolated strain had the highest homology with Proteus mirabilis（ HQ259935. 1 and KR133627. 1）. Its homology with Escherichia coli,Salmonella and Klebsiella was low. The isolated strain was pathogenic and the multi-drug resistance was strong,especially to ampicillin,mezlocillin,cephalosporin IV,cephalosporin V,cephalosporin VI,kanamycin,neomycin,tetracycline,and doxycycline. But it was sensitive to Fortum,gentamycin,Amikacin,and netilmicin. This study provides a reference for the isolation,identification and treatment of Proteus mirabilis from pigs.

关 键 词：16S RRNA 奇异变形杆菌 猪 分离鉴定 药敏试验 

分 类 号：S858.28[农业科学—临床兽医学]