急性髓系白血病HGFA、Matriptase、HAI-1、HAI-2表达水平及临床意义  

Expression and Clinical Significances of HGFA,Matriptase,HAI-1 and HAI-2 in Acute Myeloid Leukemia

作　　者：郭江睿[1] 李暐[2] 吴勇[1] 连晓岚[1] 张淑遐 陈元仲[1] GUO Jiang-Rui;LI Wei;WU Yong;LIAN Xiao-Lan;ZHANG Shu-Xia;CHEN Yuan-Zhong(Fujian Institute of Hematology,Fujian Provincial Key Laboratory of Hematology,Fujian Medical University Union Hospital,Fuzhou 350001,Fujian Province,China;School of Pharmacy,Fujian Medical University,Fuzhou 350001,Fujian Province,China)

机构地区：[1]福建省血液病研究所福建省血液病学重点实验室福建医科大学附属协和医院,福建福州350001 [2]福建医科大学药学院,福建福州350001

出　　处：《中国实验血液学杂志》2018年第4期984-992,共9页Journal of Experimental Hematology

基　　金：国家和福建省临床重点专科建设项目资助;国家自然科学基金青年项目(81001453);福建省血液医学中心建设项目资助(Min201704);福建省自然科学基金项目(2017J05127;2017J01531);福建省高校杰出青年科研人才培育项目(JA13133)

摘　　要：目的:探讨HGFA、Matriptase、HAI-1、HAI-2在急性髓系白血病患者中的表达水平及其临床意义。方法:收集91例急性髓系白血病初治患者、41例急性髓系白血病缓解患者和32例正常人的骨髓标本,利用实时荧光定量RT-PCR(qRT-PCR)法检测髓系白血病细胞HGFA、Matriptase、HAI-1、HAI-2 mRNA表达水平;比较上述基因在人急性髓系白血病患者初治组和缓解组以及健康对照组中的表达情况;分析其表达情况与其临床特征的相关性。结果:在AML初治组HGFA水平高于健康对照组(P=0.010);而HAI-2 mRNA水平则低于健康对照组(P=0.0158),但均与AML缓解组无统计学差异。HAI-1、Matriptase mRNA水平在各组中均无明显差异。在高白细胞组中HAI-2 mRNA表达较低(P=0.03902)。结论:HGF/c-Met信号系统在AML中的异常激活,有可能是其上游的调控因子HGFA表达增高和HAI-2表达降低的结果。Objective： To investigate the expression and clinical significances of HGFA,Matriptase,HAI-1 and HAI-2 in patients with acute myeloid leukemia（ AML）. Methods： The bone marrow samples from 91 AML patients,41 AML patients in complete remission,and 32 normal controls were collected. Real-time fluorescence quantitative RTPCR（ qRT-PCR） was used to detect the mRNA expressions levels of HGFA,Matriptase,HAI-1,HAI-2. The expressions of these genes were compared among AML untreated group,the complete remission group and the healthy control group. The correlation of their expression with clinical characteristics was analyzed. Results： The level of HGFA in the AML untreated group was higher than that in the healthy control group（ P〈0. 05）,while the HAI-2 mRNA level was lower than that in the healthy control group（ P〈0. 05）. The mRNA levels of HAI-1 and Matriptase were not changed significantly in all groups. The HAI-2 mRNA expression level was significantly lower in the high white blood cell group（ P〈0. 05）. Conclusion： The abnormal activation of HGF/c-Met signaling system in AML may result from the increase of HGFA expression and the decrease of HAI-2 expression of the upstream regulatory factors.

关 键 词：肝细胞生长因子激活物 丝氨酸蛋白酶Matriptase 肝细胞生长因子激活物抑制因子1 肝细胞生长因子激活物抑制因子2 急性髓系白血病 

分 类 号：R733.71[医药卫生—肿瘤]