X线诱导人鼻咽鳞状细胞癌CNE1细胞株MDR-1及P-gP的表达研究  

Expressions of MDR-1 and P-gp in human nasopharyngeal squamons cell after X-ray exposure

作　　者：皇婷 宋学薇 安书芬 王志斌 廖和和 Huang Ting;Song Xuewei;An Shufen;Wang Zhibin;Liao Hehe(Department of Oncology,Nuclear Industry 215 Hospital of Shaanxi Province,Xianyang 712000,China)

机构地区：[1]陕西省核工业二一五医院肿瘤科,咸阳712000 [2]河北省沧州市人民医院肿瘤科

出　　处：《国际肿瘤学杂志》2018年第6期321-324,共4页Journal of International Oncology

摘　　要：目的 检测X线照射前后鼻咽鳞状细胞癌CNE1细胞株中多药耐药基因（MDR）-1及P-糖蛋白（P-gp）的表达情况.方法 对CNE1细胞进行X线照射,在X线照射后、CNE1细胞培养24h后进行检测,半定量反转录-聚合酶链反应（RT-PCR）检测X线照射前后CNE1细胞中MDR-1 mRNA的表达;Western blotting检测X线照射前后CNE1细胞中P-gp的蛋白表达;共聚焦显微镜观察照射前后CNE1细胞中P-gp的蛋白表达情况.结果 RT-PCR检测结果显示,X线照射前后CNE1细胞中MDR-1mRNA的吸光度（A）值分别为0.17 ±0.01和0.34±0.03,差异具有统计学意义（t=16.541,P＜0.001）.Western blotting检测结果显示,X线照射前后CNE1细胞中P-gp蛋白的A值分别为0.02±0.01和0.04±0.01,差异具有统计学意义（t=4.612,P=0.016）.共聚焦显微镜观察X线照射后CNE1细胞中P-gp的蛋白表达绿色荧光强度高于照射前.结论 X线照射可引起CNE1细胞中MDR-1及P-gp表达的升高,提示X线照射可使鼻咽鳞状细胞癌CNE1细胞产生多药耐药性.Objective To examine the expressions of multidrug resistance gene （MDR）-1 and P-glycoprotein （P-gp） in nasopharyngeal squamous cell carcinoma CNE1 cell line before and after X-ray exposure.MethodsCNE1 cells were exposed to X-ray. After the irradiation, the CNE1 cells were cultured for 24 hours and tested. The mRNA expressions of MDR-1 in CNE1 cells were measured by semi-quantitative real-time polymerase chain reaction （RT-PCR） before and after X-ray exposure, and the protein expressions of P-gp in CNE1 cells were detected by Western blotting. The protein expressions of P-gp in CNE1 cells were observed by confocal microscope before and after X-ray exposure.Results The results of RT-PCR showed that the absorbance （A） values of MDR-1 mRNA in CNE1 cells were 0.17±0.01 and 0.34±0.03 before and after irradiation, and the difference was statistically significant （t=16.541, P〈0.001）. The results of Western blotting showed that the A values of P-gp protein in CNE1 cells were 0.02±0.01 and 0.04±0.01, and the difference was statistically significant （t=4.612, P=0.016）. The green fluorescence intensity of P-gp protein in CNE1 cells after X-ray irradiation was higher than that before X-ray irradiation by confocal microscope.Conclusion X-ray irradiation can cause the high expressions of MDR-1 and P-gp in CNE1 cells, suggesting that X-ray irradiation can induce the occurrence of multidrug resistance in CNE1 cells.

关 键 词：鼻咽肿瘤 CNE1细胞 抗药性 多药 X射线照射 

分 类 号：R739.63[医药卫生—肿瘤]