长链非编码RNA-CECR7抑制食管癌细胞增殖和凋亡的机制研究  

Mechanism of long noncoding RNA CECR7 inhibiting proliferation and apoptosis of esophageal cancer cells

作　　者：王向辉[1] 黄江平[1] 崔丰和[1] 钱海云[1] WANG Xianghui;HUANG Jiangping;CUI Fenghe;QIAN Haiyun(Department of Cardiothoracic Surgery,Jingzhou Hospital,Tongji Medical College,Huazhong University of Science and Technology,Jingzhou（434020）,China)

机构地区：[1]华中科技大学同济医学院附属荆州医院心胸大血管外科,434020

出　　处：《国际消化病杂志》2018年第4期242-246,共5页International Journal of Digestive Diseases

摘　　要：目的探讨长链非编码RNA-CECR7(lncRNA-CECR7)对食管癌细胞增殖和凋亡的影响及其可能的机制。方法荧光实时定量聚合酶链反应(qRT-PCR)检测人食管癌细胞KYSE30、EC9706、TE-13、Eca109和人食管黏膜上皮细胞HET-1A中lncRNA-CECR7的表达,以表达量最高的食管癌细胞作为转染对象,分别转染siRNACECR7(实验组)或siRNA-NC(对照组);qRT-PCR检测转染后两组中lncRNA-CECR7的表达;集落形成实验和细胞增殖试验(MTS法)检测细胞增殖能力;流式细胞术检测细胞周期和凋亡。qRT-PCR和蛋白质印迹法(Western blot)检测细胞周期蛋白依赖激酶4(CDK4)、细胞周期素D1(Cyclin D1)和p21活化蛋白激酶2(PAK2)mRNA及蛋白的表达水平。结果 lncRNA-CECR7在食管癌细胞中表达明显高于食管黏膜上皮细胞,其中TE-13细胞中的表达最高;与对照组相比,沉默CECR7后,实验组细胞周期明显受到抑制,细胞凋亡比例显著增加,细胞增殖能力被抑制。实验组TE-13细胞中CDK4、Cyclin D1和PAK2mRNA及蛋白的表达明显降低(P<0.05)。结论 lncRNA-CECR7在食管癌细胞中高表达,沉默CECR7可抑制TE-13细胞增殖,促进细胞凋亡,其机制可能是通过抑制CDK4、Cyclin D1和PAK2基因的表达,为食管癌诊断和治疗提供了新的方向。Objective This paper intends to investigate the effect of long-chain non-coding RNA-CECR7 （lncRNA-CECR7） on proliferation and apoptosis of esophageal cancer cells and its possible mechanism.Methods qRT-PCR was used to detect the expression of lncRNA-CECR7 in human esophageal cancer cell lines KYSE30, EC9706, TE-13, Eca109 and human normal esophageal mucosa epithelial cells HET-1A. The esophageal cancer cell with the highest expression of lncRNA-CECR7 was transfected with siRNA-CECR7 （experimental group） or siRNA-NC （control group）. The expression of lncRNA-CECR7 in both groups were detected by qRT-PCR. The cell proliferation was detected by using colony formation assay and MTS assay. The cell cycle and apoptosis were detected by using flow cytometry. qRT-PCR and Western blot were used to detect the mRNA and protein expression of CDK4, Cyclin D1 and PAK2.Results The expression of esophageal cancer cell lines were significantly higher than that in normal esophageal mucosa epithelial cells, of which TE-13 cells had the highest expression. Compared with the control group, after lncRNA-CECR7 was silenced, the cell cycle of the experimental group was significantly inhibited, the proportion of apoptosis increased significantly, and the cell proliferation was inhibited.Conclusions The expression of lncRNA-CECR7 was overexpressed in esophageal cancer cells. The silencing of CECR7 could inhibit the proliferation of TE-13 cells and promote the apoptosis of esophageal cancer cells. The mechanism may be through inhibiting the expression of CDK4, Cyclin D1 and PAK2 genes, which provides a new direction for the diagnosis and treatment of esophageal cancer.

关 键 词：长链非编码RNA 食管癌 细胞增殖 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]