冻鸡肉中金黄色葡萄球菌LAMP快速检测方法的建立  

Establishment of Rapid Detection Method of Staphylococcus aureus LAMP in Frozen Chicken

作　　者：袁光宇 谢体波 龚维瑶 吴紫洁 程茹 张凯 钟新敏 YUAN Guang-yu;XIE Ti-bo;GONG Wei-yao(Guizhou Kwinbon Science and Technolog for Food Safety Co.,Ltd.,Guiyang,Guizhou 55000)

机构地区：[1]贵州勤邦食品安全科学技术有限公司,贵州贵阳550009 [2]贵州安为天检测技术有限公司,贵州贵阳550009

出　　处：《安徽农业科学》2018年第25期162-163,199,共3页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技计划项目"微生物快速检测技术集成研究与应用示范";贵州省工业和信息化发展专项"技术中心能力建设";贵阳市人才创新创业项目"环介导等温扩增技术快速检测产品开发";贵阳市科技计划项目"基于大数据的农产品中毒素污染现场快速检测溯源技术应用研究"

摘　　要：[目的]建立冻鸡肉中金黄色葡萄球菌的LAMP检测方法。[方法]根据金黄色葡萄球菌特异性耐热核酸酶基因序列设计2对引物,建立LAMP检测方法,并用于检测采购自贵阳市各大超市的冻鸡肉样品。[结果]设计合成的2对引物和建立的LAMP方法检测金黄色葡萄球菌具有良好的特异性,反应体系的灵敏度为65 CFU/m L。检测采购自贵阳市各大超市和经人为污染的264份冻鸡肉样品分别用LAMP方法检测,并用国标方法检测验证,其特异性为95.2%,准确度为97.3%。[结论]该研究建立的方法为实现在冻鸡肉中金黄色葡萄球菌的快速检测奠定了良好的基础。[ Objective] The research aimed to establish a LAMP method for detection of S. aureus in frozen chicken. [ Method ] Two pairs of primers were designed based on the nuc gene sequence of S. aureus. A LAMP detection method was established a n d u s e d to detect frozen chicken samples from major supermarkets in Guiyang. [ Result] Two pairs of primers and the established LAMthe detection of S. aureus. The sensitivity was 65 CFU/^mL. 264 frozen chicken samples purchased from major supermarkets in Guiyang and polluted by humans were detected by LAMP method and verified by national standard method. The specificity was 95. 2% and the accuracy was 97. 3%. [ Conclusion] The method established in this study laid a good foundation for rapid detection of S. aureus in frozen chicken.

关 键 词：金黄色葡萄球菌 LAMP 检测方法 冻鸡肉 

分 类 号：S851.347[农业科学—预防兽医学]