卵巢浆液性肿瘤中Le^y抗原和TGF-β1的表达及相关性分析  

Study of the expression and correlation of Le^y antigen and TGF-β1 in ovarian serous tumors

作　　者：刘娟娟[1] 王圣坦[1,2] 林蓓[1] Liu Juanjuan;Wang Shengtan;Lin Bei(Department of Gynecology and Obstetrics,Shengjing Hospital of China Medical University,Liaoning Shenyang 110004,China;Department of Obstetrics and Gynecology,Hainan Provincial People＇s Hospital,Hainan Haikou 570311,China.)

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]海南省人民医院妇产科,海南海口570311

出　　处：《现代肿瘤医学》2018年第18期2921-2925,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81172491;81101527;81472437;81672590);盛京自由研究者计划项目(编号:201303)

摘　　要：目的:研究Le^y抗原与转化生长因子β1(transforming growth factor beta 1,TGF-β1)在卵巢浆液性肿瘤中的表达、相关性及临床意义。方法:应用免疫组化SP法检测68例卵巢组织(30例浆液性囊腺癌、13例交界性浆液性囊腺瘤、15例良性浆液性囊腺瘤和10例正常组织)中Le^y与TGF-β1的表达。用免疫荧光双标记法检测二者在卵巢癌组织中表达的结构关系。结果:TGF-β1在囊腺癌中的阳性率(80.00%)最高,明显高于良性瘤(66.67%)及正常组(40.00%)(P均<0.05)。Le^y表达与TGF-β1相似,Le^y在癌瘤中的阳性表达率为90.00%,明显高于交界性瘤(69.23%)、良性瘤(46.67%)及正常组(0)(P均<0.05)。TGF-β1和Le^y的表达均明显增高,二者存在正相关性(C=0.439,P<0.05)。免疫荧光结果显示TGF-β1与Le^y抗原存在空间位置上的共定位。结论:随着卵巢浆液性肿瘤的恶性进展,Le^y抗原和TGF-β1的表达上调,且两者的表达呈显著性正相关,可作为判断卵巢浆液性囊腺癌生物学行为和恶性潜能的参考指标。Objective： To detect the expression and correlation of Le^y antigen and TGF-β1 in ovarian serous tumors.Methods： Le^y antigen and TGF-β1 expression in 68 cases of ovarian tissues（ 30 cases of serous cystadenocarcinoma,13 cases of borderline serous cystadenoma and 15 cases of benign serous cystadenoma and 10 cases of normal tissue） was detected using immunohistochemical staining.Correlation between Le^y antigen and TGF-β1 was detected using immunofluorescence double labeling method.Results： The positive rate of TGF-β1 in malignant group was obviously higher than that of benign group and normal group（ P all 0.05）.The positive rate of Le^y antigen in malignant group was obviously higher than that of borderline group,benign group and normal group.There was positive correlation between Le^y antigen and TGF-β1（ C = 0.439,P 0.05）.Using the double-labeling immunofluorescence method,we observed that TGF-β1 and Le^y were located in the same position.Conclusion： With the malignant development of ovarian serous tumors,the expression of Le^y antigen and TGF-β1 are upregulated,and there are positive correlation between them.They can be used as indicators of malignant potential of ovarian serous cystadenocarcinoma.

关 键 词：卵巢浆液性肿瘤 转化生长因子Β1 Le^y抗原 免疫组化 激光共聚焦 

分 类 号：R737.31[医药卫生—肿瘤]