依达拉奉对直肠黏膜细胞放射损伤的保护作用  

The Protective Effect of Edaravone on Radiation Damage of Rectal Mucosal Cells

作　　者：王放[1] 武建军[1] 陈建兵 汪关雨 任铭[1] Wang Fang;Wu Jianjun;Chen Jianbing(Department of Radiotherapy, Peace Hospital Affilicated to Changzhi Medical Colleag)

机构地区：[1]长治医学院附属和平医院放疗科,046000

出　　处：《长治医学院学报》2018年第4期245-247,共3页Journal of Changzhi Medical College

基　　金：长治医学院科研启动基金项目(普及项目)(QDZ201507)

摘　　要：目的:探讨依达拉奉对体外培养的人正常直肠黏膜上皮细胞放射损伤的保护作用。方法:体外培养人正常直肠黏膜上皮细胞(HMC)至对数生长期,分为对照组和处理组,对照组细胞给予单纯培养液培养,处理组细胞培养液中加入100mol/L依达拉奉,分别经2Gy和6Gy X线照射后,采用流式细胞仪检测各组的细胞凋亡率,SOD试剂盒和MDA试剂盒分别检测各组SOD活性和MDA含量的变化。结果:经2Gy和6Gy X线照射后,处理组细胞凋亡率低于对照组凋亡率,差异有统计学意义(P<0.05),处理组2Gy组、6Gy组细胞SOD活性分别为2.78±0.05、2.90±0.02,对照组2个剂量组细胞SOD活性分别为2.54±0.03、2.30±0.21,处理组高于对照组,差异均具有统计学意义(P<0.05);处理组中2Gy组、6Gy组细胞MDA含量分别为0.11±0.04、0.89±0.15,对照组2个剂量组细胞MDA含量分别为2.22±0.15、1.48±0.11,处理组低于对照组,差异均具有统计学意义(P<0.05)。结论:依达拉奉能够降低直肠黏膜上皮细胞的放射损伤程度,对直肠黏膜上皮细胞具有明显的放射损伤保护作用。Objective：To investigate the protective effect of edaravone on radiation injury of human normal rectal mucosal epithelial cells（HMC）.Methods：HMC were cultured in vitro to logarithmic growth period,which was divided into control group,radiation group and administration group.After being irradiated by 2 Gy and 6 GyX-ray,flow cytometer was used to detect apoptosis of HMC and special kit was used to detect MDA content and SOD activity.Results：Ionizing radiation could induce apoptosis of HMC in each group,and the apoptosis rate of cells was related to the dose of ionizing radiation（P〈0.05）.Compared with the control group,the experimental group of different doses had different cell apoptosis rates（P〈0.05）,and the HMC of experimental group decreased SOD activity and increased MDA content after irradiation（P〈0.05）.Compared with HMC without edaravone pretreatment,the SOD activity was higher and the MDA content was lower after HMC pretreatment with edaravone（P〈0.05）.Conclusion：Edaravone can reduce the degree of radiation damage of epithelial cells of HMC,and has obvious protective effect on the radiation damage of epithelial cells of HMC.

关 键 词：依达拉奉 人直肠黏膜上皮细胞 放射损伤 自由基 

分 类 号：R574.6[医药卫生—消化系统]