盐酸法舒地尔对脑出血大鼠脑血管痉挛及炎性细胞因子的影响分析  

The effect of Hydrochloride fasudil on vascular spasm and inflammatory cytokines in rats with cerebral hemorrhage

作　　者：黄琼 姚胜旗[1] 许敏[1] 朱筱琪 刘枫狄 HUANG Qiong;YAO Sheng-qi;XU Min(Department of Neurology,Tongren Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200336,China)

机构地区：[1]上海市同仁医院/上海交通大学医学院附属同仁医院神经内科,上海200336 [2]国药集团上海血液制品有限公司,上海200051

出　　处：《临床和实验医学杂志》2018年第18期1914-1918,共5页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨盐酸法舒地尔对脑出血大鼠脑血管痉挛及大鼠炎性细胞因子的影响。方法清洁级SD大鼠160只,随机分为对比A组、对比B组、观察A组、观察B组,各40只,对比A组为正常对照,不进行任何处理,对比B组进行假手术;观察A组建立脑出血模型,观察B组在建立脑出血模型后12 h使用盐酸法舒地尔。建模后再随机将各组大鼠按照术后1 d、3 d、7 d、14 d分为4个亚组,各10只,测定各组术后神经功能缺损评分、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α),并做基底动脉病理学检查。结果术后3 d、7 d、14 d观察B组神经功能评分均明显低于观察A组(P<0.05)。观察A组和观察B组血清IL-6在术后1 d达高峰,TNF-α在术后3 d达高峰,在术后14 d逐渐降低。观察B组各时间段血清IL-6、TNF-α均低于观察A组(P<0.05),术后14 d观察B组与对比A值、对照B组血清IL-6、TNF-α差异无显著性(P>0.05)。术后1 d、3 d时各组基底动脉管腔面积与血管面积比值:观察A组和观察B组均明显低于对比A组和对比B组,观察A组比值低于观察B组(P<0.05),术后7 d、14 d观察A组略低于观察B组,但在术后14 d时两组差异无显著性(P>0.05),观察A组和观察B组术后1 d、3 d、7 d基底动脉管壁厚度与血管外径周长比值均明显高于对比A组和对比B组(P<0.05),观察B组比值在术后1 d、3 d、7 d均低于观察A组(P<0.05)。结论盐酸法舒地尔可减轻脑出血大鼠脑血管痉挛,并降低大鼠血清IL-6、TNF-α含量。Objective To discusses the effect of Hydrochloride fasudil on vascular spasm and inflammatory cytokines in rats with cerebral hemorrhage. Methods A total of 160 grade clean SD rats were randomly divided into contrast group A,contrast group B,observation group A and observation group B with 40 rats in each group. Contrast group A was designed as normal control group without any processing,contrast group B was sham-operated group,observation group A was made into cerebral hemorrhage model,12 h after cerebral hemorrhage model was established,observation group B was given Hydrochloride fasudil. After modeling in postoperative 1 d,3 d,7 d and 14 d they were divided into 4 subgroups with 10 in each subgroup. The neural function defect scale,IL-6,TNF-α were detected and basal artery pathological examination was performed. Results 3 d,7 d,14 d after operation,nerve function score of observation group B were significantly lower than those of observation group A（P〈0. 05）. Serum IL-6 in observe group A and B peaked in postoperative 1 d,TNF-α peaked in postoperative 3 d and gradually decreased in postoperative 14 d. Serum IL-6 and TNF-α in observation group B were lower than those of observation group A（P〈0. 05）,14 d after surgery,serum IL-6,TNF-α in observation group B and contrast group B and A had no significant difference（P〉0. 05）. Postoperative1 d,3 d,basilar artery lumen area with vascular area ratio： observation group A and group B were significantly lower than those of contrast group A and B,observation group A ratio were lower than observation group B（P〈0. 05）. 7 d and 14 d after surgery,observation group A were slightly lower than group B,but 14 d after surgery they had no significant difference（P〉0. 05）. In observation group A and B after 1 d,3 d,7 d,basilar artery wall thickness and the vascular diameter circumference ratio were significantly higher than that of contrast group A and B（P〈0. 05）.The observation group B ratio in postoperative 1 d,3 d,7 d were lower than thos

关 键 词：大鼠 脑出血 盐酸法舒地尔 脑血管痉挛 炎性细胞因子 

分 类 号：R743.34[医药卫生—神经病学与精神病学]