阿司匹林对慢性应激抑郁大鼠海马细胞因子表达的影响  

Influence of aspirin for the expression of cytokines in hippocampus of chronic stress depression rats

作　　者：唐文诚 李敏[1] 易辉燕 罗芳芳 杨丽[1] Tang Wencheng;Li Min;Yi Huiyan;Luo Fangfang;Yang Li(The Third Hospital of Mianyang · Sichuan Mental Health Center, Mianyang 621000, China)

机构地区：[1]绵阳市第三人民医院.四川省精神卫生中心,四川绵阳621000

出　　处：《四川精神卫生》2018年第4期313-317,共5页Sichuan Mental Health

基　　金：四川省卫生厅科研课题(120320)

摘　　要：目的观察阿司匹林对抑郁模型大鼠海马组织内白介素1β(IL-1β)、白介素4(IL-4)、白介素6(IL-6)、白介素10(IL-10)及肿瘤坏死因子α(TNF-α)表达的影响,探讨阿司匹林潜在的抗抑郁作用和机制。方法选取成年雄性SD大鼠30只,按随机数字表法分为正常组、模型组和阿司匹林组各10只,正常组在原条件下饲养,模型组和阿司匹林组采用慢性不可预见性温和刺激(CUMS)结合孤养方式建立抑郁大鼠模型。建模后,阿司匹林组给予阿司匹林灌胃,正常组和模型组给予等体积生理盐水灌胃,每天1次,连续21 d。灌胃21 d后,通过旷场实验、强迫游泳实验和高架十字迷宫实验评定大鼠行为学,采用酶联免疫吸附试验(ELISA)检测大鼠海马内IL-1β、IL-4、IL-6、IL-10及TNF-α水平。结果干预后,阿司匹林组的水平和垂直运动次数多于模型组,不动时间短于模型组,进入开放臂次数、进入迷宫总次数多于模型组,进入开放臂时间百分比高于模型组,差异均有统计学意义(P<0.05或0.01);阿司匹林组IL-10水平高于模型组,TNF-α和IL-6水平均低于模型组(P<0.05或0.01)。结论阿司匹林可改善抑郁模型大鼠的抑郁行为,其可能是通过细胞因子发挥抗抑郁作用。Objective To observe the influence of aspirin on the expressions of interleukin-1β（ IL-1β）,interleukin-4（ IL-4）,interleukin-6（ IL-6）,interleukin-10（ IL-10）,and tumor necrosis factor-α（ TNF-α） in hippocampus tissue of depressive model rats,and to explore the potential antidepressant effect and mechanism of aspirin. Methods 30 male SD rats were divided into normal group,model group and aspirin group according to random number table method,each group consists of 10 rats.The normal group was fed under the original condition. The model group and aspirin group were given chironic unpredictable mild stress（ CUMS） combined with solitary rearing to establish depression rat model. After modeling,aspirin group was given aspirin by gavage,normal group and model group were given the same volume of normal saline by gavage,once a day for 21 days. After gavage for 21 days,the behavioristics of all rats was assessed by open field test,forced swimming test and elevated plus maze test. The levels of IL-1β,IL-4,IL-6,IL-10 and TNF-α in hippocampus tissue were detected by enzyme-linked immunosorbent assay（ ELISA）. Results After intervention,the times of horizontal and vertical movement in aspirin group was more than that in model group,and the immobility time was shorter than that in model group. The times of entering open arms,total number of times for entering the maze in aspirin group was more than that in model group,the percentage of time into the open arm was higher than that in model group,the differences were statistically significant（ P 〈 0. 05 or 0. 01）. The level of IL-10 in aspirin group was higher than that in model group,while the levels of TNF-α and IL-6 were lower than those in model group（ P 〈 0. 05 or 0. 01）. Conclusion Aspirin can ameliorate the depressive behavior for depression model rats,which may play an antidepressant role through cytokines.

关 键 词：阿司匹林 抑郁 慢性不可预见性温和应激 白介素1Β 白介素4 白介素6 白介素10 肿瘤坏死因子α 

分 类 号：R749.4[医药卫生—神经病学与精神病学]