超高效液相色谱-串联质谱法同时测定水中的呋喃丹、莠去津和微囊藻毒素  

Simultaneous determination of carbofuran, atrazine and microcystins-LR in water by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：孙艳芳[1] 张剑峰[1] 王缘[1] 任淑艳[1] 付宇[1] SUN Yan-Fang;ZHANG Jian-Feng;WANG Yuan;REN Shu-Yan;FU Yu(Heilongjiang Province Center for Disease Control and Prevention,Harbin 150030,China)

机构地区：[1]黑龙江省疾病预防控制中心,哈尔滨150030

出　　处：《食品安全质量检测学报》2018年第15期4050-4054,共5页Journal of Food Safety and Quality

摘　　要：目的建立同时测定水中的呋喃丹、莠去津和微囊藻毒素-LR的超高效液相色谱-串联质谱方法。方法样品直接过0.22μm的水系滤膜,以0.1%甲酸水溶液(A)和乙腈(B)为流动相经BEH C18柱(100 mm×2.1 mm,1.7μm)梯度洗脱分离,串联四极杆质谱在电喷雾正离子(electrospray ionization,ESI+)模式下同时测定呋喃丹、莠去津和微囊藻毒素-LR的含量。结果呋喃丹、莠去津和微囊藻毒素-LR在0.5~50 ng/m L范围内线性良好,r≥0.999,平均加标回收率90.2%~99.5%,精密度为2.1%~4.5%。结论本方法操作简单、灵敏度高、线性范围宽、结果准确可靠,可用于水中的呋喃丹、莠去津和微囊藻毒素-LR的同时测定。Objective To establish a method for simultaneous determination of carbofuran, atrazine and microcystins-LR in water by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）. Methods The samples were directly filtered 0.22 μm water filtration membrane, and then were separated with gradient elution through the BEH C18 column（100 mm×2.1 mm, 1.7 μm） with the mobile phase of 0.1% formic acid aqueous solution（A） and acetonitrile（B）. The carbofuran, atrazine and microcystins-LR were determined simultaneously by UPLC-MS/MS in electrospray positive ion（ESI＋） mode. Results Carbofuran, atrazine and microcystins-LR had good linear relationships in the range of 0.5-50 ng/m L, and the correlation coefficients were larger than 0.999, the average recoveries were 90.2%-99.5% and the precisions were 2.1%-4.5%. Conclusion The method has the advantages of simple operation, high sensitivity, wide linear range, accurate and reliable results, which can be used for simultaneous determination of carbofuran, atrazine and microcystins-LR in water.

关 键 词：水 呋喃丹 莠去津 微囊藻毒素-LR 超高效液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学] X832[理学—化学]