维生素E琥珀酸酯联合5-氟尿嘧啶和5′-脱氧氟脲苷促进人结肠癌细胞凋亡  

The effect of Vitamin E succinate combined with 5-fluorouracil and 5′-deoxyfluoride induced apoptosis in human colon cancer cells

作　　者：王绮雯[1] 张继民[2] 李爱群[3] 邹湘才[2] WANG Qiwen;ZHANG Jimin;LI Aiqun;ZOU Xiangcai(Department of Surgery Labo-ratory,The Second Affiliated Hospital of Guangzhou Medical Uaiversity,Guangzhou 510260,China)

机构地区：[1]广州医科大学附属第二医院外科实验室,广州510260 [2]广州医科大学附属第二医院胃肠外科,广州510260 [3]广州医科大学附属第二医院心研所,广州510260

出　　处：《实用医学杂志》2018年第16期2638-2643,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81071883);广东省医学科研基金(编号:A2018406)

摘　　要：目的探讨维生素E琥珀酸酯(VES)联合5-氟尿嘧啶(5-FU)和5′-脱氧氟脲苷(5′-DFUR)对人结肠癌细胞株LS174T凋亡的影响。方法 LS174T细胞常规培养,分6组(0,20μmol/L VES,1μmol/L 5-FU,2μmol/L 5′-DFUR,20μmol/L VES+1μmol/L 5-FU,20μmol/L VES+2μmol/L 5′-DFUR),处理24 h,倒置显微镜观察细胞形态,MTS法检测细胞的增殖情况;Western blot法检测细胞bid、bax、bcl-2蛋白的表达情况;流式细胞仪技术检测细胞凋亡情况。结果 VES分别与5-FU、5′-DFUR联合能够抑制人结肠癌细胞的增殖,增强bid、bax蛋白的表达,降低bcl-2蛋白的表达,促进细胞发生凋亡。结论 VES分别与5-FU、5′-DFUR联合作用时能抑制细胞增殖,诱导细胞凋亡,其中VES+5′-DFUR联合作用效果最为显著。这可能与细胞中促凋亡蛋白bid、bax表达的增加、抗凋亡蛋白bcl-2表达的降低有关。Objective To study the effect of vitanfin E succinate （VES） combined with 5-fluorouracil （ 5-FU ） and 5＇-Deoxy-5-fluorouridine （ 5＇-DFUR ） on the apoptosis of human colon cancer cell line LS174T in vitro. Methods LS174T cells were routinely cultured in 6 groups （0,20 μmol/L VES, 1 μmol/L 5-FU, 2 μmol/L 5＇-DFUR, 20 μmol/L VES ＋ 1 μmol/L 5-FU, 20 μmol/L VES ＋ 2 μmol/L 5＇-DFUR） for 24 h. The morphology of cell was observed by inverted microscope, cell proliferation was detected by MTS ; expression of bid, Bax, bcl-2 protein were detected by Western blot ; flow cytometry technique to detect apoptosis. Results VES combined with 5-FU, 5＇-DFUR inhibited the proliferation of human colon cancer cells, enhanced the expression of bid, Bax protein, reduced the expression of Bcl-2 protein and promoted apoptosis. Conclusion VES combined with 5-FU, 5＇-DFUR can inhibit cell proliferation and induce apoptosis, and the effect of VES＋5 ＇-DFUR is the most signifi- cant. This may be related to the increase of the expression of Pro apoptotic protein bid, Bax and the expression of anti apoptotic protein Bcl-2 in cells.

关 键 词：维生素E琥珀酸酯 5-氟尿嘧啶 5'-脱氧氟脲苷 LS174T 凋亡 

分 类 号：R735.3[医药卫生—肿瘤]