不同正畸力对大鼠磨牙牙根发育的影响  

Different orthodontic forces on the root development of rat molars

作　　者：李晓光[1] 刘士臣[2] 肖媛媛[1] 杨东红[1] 孔宇[1] 刘继光[3] LI Xiao-guang;LIU Shi-chen;XIAO Yuan-yuan;YANG Dong-hong;KONG Yu;LIU Ji-guang(Stomatology school of Jiamusi University,Jiamusi 154007)

机构地区：[1]佳木斯大学附属口腔医院正畸科,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院骨外科,黑龙江佳木斯154007 [3]佳木斯大学研究生处,黑龙江佳木斯154007

出　　处：《牙体牙髓牙周病学杂志》2018年第8期450-454,470,共6页Chinese Journal of Conservative Dentistry

基　　金：黑龙江省卫生计生委科研课题(2016-285);佳木斯大学科学技术重点项目(12Z1201509)

摘　　要：目的:探讨不同大小的正畸力对大鼠磨牙牙根发育的影响。方法:取5周龄健康雄性SD大鼠96只,随机分为3个实验组和1个空白对照组(n=24)。对3个实验组大鼠建立正畸模型后,分别对其施加50(A组)、75(B组)、100 g(C组)的正畸力;空白对照组大鼠不做任何处理。分别于正畸加力后1、3、7、14 d各时间点每组随机处死6只大鼠,取其左侧上颌骨并拍摄CBCT,比较大鼠第一磨牙近中根的长度;然后对颌骨进行连续切片后,进行HE染色及BSP、MMP-9免疫组化染色。结果:CBCT测量结果显示,与空白对照组相比,第一磨牙近中根长度B组在加力后14 d时及C组在加力后7、14 d时均有统计学差异(P<0.05)。免疫组化染色结果显示,与空白对照组相比,B组在加力后14 d时BSP表达下调(P<0.05),而在7 d时MMP-9表达开始上调(P<0.05);C组在加力7 d时BSP表达下调、14 d时达到峰值(P<0.05),从加力3 d时,MMP-9随着加力时间的延长其表达水平逐渐升高(P<0.05)。结论:BSP及MMP-9的表达均与正畸力值大小及加力时间相关,其中100 g的正畸力对大鼠牙根发育影响较大。AIM： To investigate the effects of orthodontic force on root developmenl of rat molars. METH- ODS ： 5 - week - old healthy male SD rats were used for the establishment of orthodontic models and treated with the or- thodontic force of 50 g（group A）, 75 g（ group B）, 100 g（ group C） and 0 g（the control, goup D） respectively. The rats were sacrificed at 1 d,3 d,7 d and 14 d respectively（n =6）. After CBCT photography, HE staining, bone sialoprotein （BSP） and MMP-9 immunohistochenfical staining were performed. RESULTS ： CBCT results showed that the root length of the first molars of group B was longer thin1 that of group D（P 〈0.05） at 7 and 14 days. BSP and MMP -9 analysis results showed in group B, the expression of BSP was down - regulated （ P 〈 0.05 ） at 14 days after treatment, while the expression of MMP-9 was up-regulated（P 〈0.05） at 7 days. In group C, the expression of BSP was down -regulated （P〈0.05） on the 7th day after adding force until 14 days, but the expression of MMP -9 was up -regulated （P 〈0.05） at the 3rd day. The gray value of group C was significanlly higher than that of the control group（P 〈0.05）. CONCLUSION ： 100 g orthodontic force have a greater effect on the root development of rats in a time - dependent manner.

关 键 词：正畸力 牙根发育 CBCT 骨涎蛋白(BSP) MMP-9 

分 类 号：R783.3[医药卫生—口腔医学]