不同孔径支架材料对施万细胞增殖的影响研究  

Effects of different pore size scaffolds on proliferation of Schwann cells

作　　者：唐鹏 王铮 杨倩[3] 王春梅[2] 王安庆[1] 刘四海[1] 王飞[1] 崔志刚[1] 韩新祚[1] 顾蕊[1] 亓攀 段春光 Tang Peng;Wang Zheng;Yang Qian;Wang Chunmei;Wang AnQin;Liu Sihai;Wang Fei;Qi pan;Gu Rui;Cui Zhigan;Duan Chunguang(Department of Orthopedics and Rehabilitation,China Rehabilitation Research Center,Beijing Charity Hospital,Belting 100068,China)

机构地区：[1]中国康复研究中心北京博爱医院骨与关节康复科,北京100068 [2]第四军医大学附属西京医院创伤骨科 [3]第四军医大学附属西京医院超声科 [4]深圳大学总医院

出　　处：《中华生物医学工程杂志》2017年第4期265-270,共6页Chinese Journal of Biomedical Engineering

基　　金：全军医学青年基金项目（13QNP130）;陕西省自然科学基金青年项目（2015JM8411）

摘　　要：目的 探讨支架材料本身的结构变化对神经细胞增殖的影响.方法 选择1～3d新生SD大鼠的坐骨和臂丛神经进行施万细胞（SC）原代培养及纯化.应用滴注法将纯化后的施万细胞接种在不同孔径的聚丙交酯-乙交酯共聚物（PLGA）与磷酸三钙（TCP）混合的支架材料上,观察施万细胞的增殖情况.通过形态学及分子生物学检测观察.结果 大孔径组的细胞增殖为0.43±0.01,小孔经组的细胞增殖为0.38±0.01.细胞增殖曲线显示48 h后大孔径组的细胞增殖明显高于小孔径组（JP＜0.05）.ELISA检测结果显示48 h,大孔径组神经生长因子表达的OD值为0.66±0.01;小孔径组神经生长因子表达的OD值为0.71±0.01（P＜0.05）.结论 小孔径支架材料早期虽然刺激了细胞的增殖,最终,施万细胞的细胞生物学行为受到抑制.大孔径支架材料虽然早期细胞基数不足,但因支架材料三维空间结构足够容纳细胞,使细胞数量逐步增加.因此,支架材料的不同三维空间结构可直接影响神经细胞在支架材料上的增殖.Objective To investigate the effects of structural changes of scaffolds on the proliferation of nerve cells.Methods The primary cultured and purified Schwann cells （SCs） were included from the sciatic and brachial plexus nerve of 1-3 days oldfetal SD rats.The purified SCs were inoculated on the scaffolds with different pore sizes by the drip method to determine the proliferation of SCs.Morphology and molecular biology were used for the examination.Results The cell proliferation in the large aperture group was significantly better than that in the small aperture group.The CCK-8 cell proliferation curve showed thatthe cell proliferation at 96 h in the large aperture group （0.43±0.01）was significantly higher than that in the small aperture group （0.38±0.01）（P〈0.05）.The findings of ELISA showed that the expression of nerve growth factor in the large aperture group was significantly higher than that in the small aperture group （P〈 0.05）.Conclusion Although the small aperture scaffoldmaterial stimulates the proliferation of cells at the early stage,the biological behavior of SCs was inhibited.Although the early cell base of the large aperture scaffoldmaterial is insufficient,the three-dimensionalspatial structure of the scaffold material is enough to accommodate cells,so that the number of cells gradually increases.Thus,the different three-dimensional spatial structure of the scaffold material may directly affect the proliferation of nerve cells on the scaffold material.

关 键 词：组织工程 细胞骨架 施万细胞 

分 类 号：R151.2[医药卫生—营养与食品卫生学]