聚吡咯/聚乳酸生物材料联合骨髓基质细胞移植治疗脊髓损伤的基础研究  

Experimental research on PPy/PLA/BMSCs to treat spinal cord injury

作　　者：Raynald 舒兵[3] 黄华[4] 周俊峰 孙晓丹[5] 秦川[1] 安沂华[2,3] Raynald1,2 , SHU Bing3, HUANG Hua5 , ZHOU Junfeng4, SUN Xiaodan4, QIN Chuan1, AN Yihua2,3.(1. Institute Laboratory Animal Science, CAMS＆PUMC, Beijing 100021 ,China; 2. The General Hospital of the Chinese People＇ s Armed Police Forces, Beijing 100039; 3. Capital Medical University-, Beijing Sanbo Brain hospital, Beijing 100050; 4. School of Materials Science and Engineering, TsinghuaUniversity, Beijing 100084; 5. Capital Medical University, Beijing Neurosurgical Institute, Beijing 10005)

机构地区：[1]中国医学科学院医学实验动物研究所,北京100021 [2]中国人民武装警察部队总医院,北京100039 [3]首都医科大学北京三博脑科医院,北京100093 [4]首都医科大学北京市神经外科研究所,北京100050 [5]清华大学材料学科与工程系,北京100084

出　　处：《中国比较医学杂志》2018年第8期32-37,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(81571165)

摘　　要：目的研究聚吡咯(polypyrrole,PPy)/聚乳酸(PLA)/骨髓基质细胞(bone marrow stromal cells,BMSCs)复合治疗脊髓损伤的可行性,并探讨其可能机制。方法制备聚吡咯/聚乳酸支架材料。从大鼠骨髓中分离、培养BMSCs;制作大鼠脊髓全横断模型,在T8水平脊髓头尾端完全横断脊髓,用虹膜剪刀去除长度约2.5 mm的脊髓组织。根据植入材料的不同将实验分为PPy/PLA组、PPy/PLA/BMSCs组及损伤组,损伤组无材料及细胞植入。术后6周处死大鼠,观察不同移植组体内轴突和血管生长情况。结果免疫荧光、电镜及q PCR结果证实复合移植可以显著促进轴突和血管生长,同时也提供了神经保护作用。结论 PPy/PLA/BMSCs可以促进大鼠脊髓损伤修复,其机制可能包括材料支架作用和BMSCs在体内促进血管生成,改善局部的微环境,因此促进新轴突生长而保护局部的神经元。Objective To investigate the feasibility of using polypyrrole/poly（ lactic acid）（ PPy/PLA） combined with bone marrow stromal cells to treat spinal cord injury. Methods The PPy/PLA scaffold material was prepared.BMSCs were extracted from rat bone marrow and cultured and expanded in vitro. Rat spinal cord was completely transected,after which PPy/PLA or PPy/PLA/BMSCs were transplanted. Six weeks later,rats were sacrificed,the growth of axons and blood vessels of the different groups was analyzed,and the therapeutic effects of cells,materials,and the combined transplantation were assayed. Results Immunofluorescence and electron microscopy showed that the combination of PPy/PLA/BMSCs not only promoted axonal and vascular growth,but also provided neuroprotective effects. Conclusions PPy/PLA/BMSC combined therapy can repair injured nerves after spinal cord injury. Based on these result,we conclude that PPy/PLA and BMSCs can provide axonal regeneration and neuroprotection by promoting vascular formation.

关 键 词：聚吡咯/聚乳酸 骨髓基质细胞 脊髓损伤 移植 

分 类 号：R-33[医药卫生]