雪貂微卫星DNA遗传多样性分析  

Genetic diversity analysis of laboratory domestic ferret microsatellite DNA

作　　者：刘先菊[1] 滕永康[1] 丛日旭 陈敏 张旭 向志光[1] 滕庆峰 刘云波[1] LIU Xianju1 , TENG Yongkang1, CONG Rixu1, CHEN Min2, ZHANG Xu1 , XIANG Zhiguang1, TENG Qingfeng1, LIU Yunbo1.(1. Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Beijing 100021, China; 2. Beijing HFK Bioscience Co. ,LTD, Beijing 102200, Chin)

机构地区：[1]中国医学科学院医学实验动物研究所,北京100021 [2]北京华阜康生物科技股份有限公司,北京102200

出　　处：《中国比较医学杂志》2018年第8期43-48,共6页Chinese Journal of Comparative Medicine

基　　金：国家重点研究计划(2017YFA0105201)

摘　　要：目的筛选、优化雪貂微卫星DNA引物,评估、分析雪貂种群的遗传多样性。方法采用21个雪貂微卫星位点,对随机抽取的30只雪貂血液样本进行基因组DNA提取及PCR扩增,PCR产物经2%Agrose电泳和6%PAGE电泳鉴定后进行STR扫描检测,用Popgene1.32软件对STR扫描结果进行数据处理和分析。结果 21个微卫星标记均呈现出遗传多样性,共检测到49个等位基因,观测等位基因数1~4个,平均2.3个;有效等位基因数1.000~3.750个,平均1.6685个;观察杂合度0~0.7333,平均0.3216;期望杂合度0~0.6424,平均0.3394;香隆指数0~1.1773,平均1.0768;多态信息含量0~0.6100,平均0.5485。结论本文筛选的21对雪貂微卫星引物,通过微卫星扫描分析验证,具有稳定的PCR扩增,微卫星标记均表现为多态性,并处于期望数值范围,没有显著地偏离Hardy-Weinberg平衡期望值。Objective To screen and optimize microsatellite primers of the laboratory domestic ferret and analyze its population genetic diversity. Methods A total of 30 domestic ferrets were randomly chosen and their genomic DNA was extracted from blood using Easy Pure Blood Genomic DNA Kit; subsequently,microsatellite DNA was amplified by standard polymerase chain reaction. The products of amplification were tested by STR scanning after 2% agarose gel and6% PAGE electrophoresis. Data processing and genetic analysis were completed using Popgene 1. 32 software. Results A total of 21 pairs of microsatellite loci showed genetic polymorphism and 49 alleles were detected. The number of alleles was1-4,with an average of 2. 3. The average observed heterozygosity was 0. 3216,the average expected heterozygosity was0. 3394,the average Xianglong index was 1. 0768,and polymorphic information was 0. 5485. Conclusions We analyzed21 pairs of microsatellite primers for laboratory domestic ferret,PCR amplification steady,microsatellite primers were shown as polymorphism and in the expected value range,and there was no significant deviation from Hardy-Weinberg equilibrium.

关 键 词：雪貂 微卫星DNA 遗传多样性 

分 类 号：R-33[医药卫生]