广西地区孕妇巨细胞病毒感染产前筛查与产前诊断分析  

Analysis of prenatal screening and diagnosis of cytomegalovirus infection in pregnant women in Guangxi

作　　者：李东明[1] 佘尚扬[1] 陈巧佩[1] 赵江阳 LI Dong-ming;SHE Shangyang;CHEN Qiaopei;ZHAO Jiang-yang(Materna and Child Health Care Hospital of Guangxi Zhuang Autonomous Region,Nanning 530000,China)

机构地区：[1]广西壮族自治区妇幼保健院,南宁530003

出　　处：《中国优生与遗传杂志》2018年第7期74-75,73,共3页Chinese Journal of Birth Health & Heredity

基　　金：广西壮族自治区卫生和计划生育委员计划课题(Z2016108)

摘　　要：目的了解广西地区孕妇巨细胞病毒(CMV)血清流行率与产前诊断孕妇胎儿样本CMV阳性率,探讨孕期CMV感染与不良妊娠结局的相关性。方法对80 318例产前检查孕妇采用酶联免疫吸附试验(ELISA)检测静脉血CMV-IgM,依据临床不良妊娠情况分组;14 447例孕妇依据孕周取脐带血、羊水和绒毛进行产前诊断,采用聚合酶链反应(PCR)检测CMVDNA,依据是否有异常超声表现分组。结果孕妇静脉血CMV-IgM总阳性率为1.15%,不良妊娠或妊娠史组阳性率为2.25%,早、中和晚孕期阳性率分别为0.90%、1.27%和1.59%,正常组阳性率为1.05%,早、中和晚孕期阳性率分别为1.47%、2.74%和3.76%,2组间比较差异有统计学意义(P<0.05)。产前诊断样本CMV-DNA总阳性率为0.39%,脐带血、羊水和绒毛CMVDNA阳性率分别为0.34%、0.49%和0.19%;不良妊娠组CMV-DNA总阳性率为0.53%高于正常组的0.12%(P<0.05)。结论广西地区孕妇CMV感染比例较高,有一定的母婴传播率,CMV感染与不良妊娠结局相关。Objective：To investigate the seroprevalence of cytomegalovirus（CMV）in pregnant women and the vertical transmission.and to further clarify the relationship of adverse pregnancy outcomes of CMV infection in pregnant women.Methods：CMV-IgM of serum was tested by enzyme-linked immunosorbent assay（ELISA）in 80 318 pregnant women. According to the gestational weeks,Umbilical cord blood,amniotic fluid and villus tissue collected from 14 447 pregnant women,which were used to detect CMV-DNA by PCR method.Results：The positive rate of CMV-IgM was 1.15% in pregnant women. The positive rate of CMV-IgM was 2.25% in adverse pregnant women,and it was 0.90%,1.27% and 1.59% in the first trimester,in the second trimester and in the third trimester of pregnancy respectively. The positive rate of CMV-IgM was higher than that（1.05%）in healthy group（P〈0.05）,and it was 1.47%,2.74% and 3.76% in the first thrimester,in the second thrimester and in the third thrimester of pregnancy respectively. The positive rate of CMV-DNA was 0.42% in preganat women. The positive rate of CMVDNA was 0.39% in prenatal diagnostic sample,and it was 0.34%,0.49% and 0.19% in cord blood,amniotic fluid and villus tissue respectively. The positive rate of CMV-NDA（0.53%）in adverse pregnant women was higher than that（0.12%）in healthy group（P〈0.05）. Conclusion：The rate of CMV infection in pregnant women was high in Guangxi. There was some vertical transmission. CMV infection were associated with adverse pregnancy outcomes.

关 键 词：巨细胞病毒 孕妇 产前筛查 产前诊断 

分 类 号：R714.55[医药卫生—妇产科学]