黄体酮对大鼠视神经夹伤后视神经节细胞保护作用的研究  

Protective effect of progesterone on rat optic nerve after optic nerve crush

作　　者：孙海燕[1] 孙文婷 安国禹 赵平[1] Sun Haiyan;Sun Wenting;An Guoyu;Zhao Ping(Third Hospital of Hebei Medical University,Shijiazhuang 050051,China）Hospital of Xianxian Traditional Chinese Mech＇cine,Cangzhou 062250,China)

机构地区：[1]河北医科大学第三医院眼科,石家庄050051 [2]献县中医院眼科,沧州062250

出　　处：《中国实用眼科杂志》2017年第8期836-840,共5页Chinese Journal of Practical Ophthalmology

摘　　要：目的 探讨黄体酮对大鼠视神经钳夹伤后视神经的保护作用.方法 清洁级健康雄性SD大鼠50只随机分为3组：正常组10只（20眼）;治疗组20只,右眼制备成视神经不完全损伤模型,1h后开始给予10 mg/kg黄体酮（progesterone,PROG）注射液腹腔注射,每d1次,至实验结束;对照组给予等量蒸馏水腹腔注射;按伤后1、3、7、14、28 d分五个时间点,各时间点每组分别取4只眼的视网膜,用予HE染色观察视网膜的形态学变化和检测胰岛素样生长因子-1（Insulin-like growth factor-1,IGF-1）及水通道蛋白-4（Aquaporin-4,AQP-4）的平均光密度值.结果 在损伤后的1、3、7d对照组IGF-1的表达均较正常组升高,具有统计学意义（P＜0.05）,7d后表达下降,14 d时接近于正常组.治疗组与对照组相比,在损伤后各时间点IGF-1的阳性表达均明显增强,具有统计学意义（P＜0.05）.对照组AQP-4在损伤后1d表达开始升高,3d时表达最高,7d开始下降,14 d仍高于正常组,具有统计学意义（P＜0.05）,28 d时接近于正常组.治疗组在1、3、7、14 d AQP-4的阳性表达较对照组均明显降低,但高于正常组,具有统计学意义（P＜0.05）.结论 大鼠视神经不完全损伤后视网膜组织中IGF-1和AQP-4表达增强,参与了视神经损伤的过程.PROG能够降低大鼠视神经不完全损伤后视网膜组织中AQP-4的表达,升高视网膜组织中IGF-1的表达,对视神经节细胞有一定保护作用.Objective To observe protective effect of progesterone on rat Optic Nerve after optic nerve crush.Methods Fifty female SD rats were randomly divided into three groups,normal group with 10 rats （20 eyes,the normal group had no treatment）,control group with 20 rats and treatment group with 20 rats.Mis-classification damage analogues were made on control group and treated group.For the treatment group,progesterone was injected to the rats＇ abdominal cavity （10mg/kg） after one hour of injury,for once a day and until the end of experiment.For the control group,distilled water was injected to the rats＇ abdominal cavity （10mg/kg） after one hour of injury,for once a day and until the end of experiment.Four optic retinas of every group were taken.Fixed,embedded,sliced for HE staining to observe the morphological changes of the retina and detect the mean OD value of IGF-1 and AQP-4.Results The expression of IGF-1 in control group enhanced significantly after 1 day,3 days and 7days of injury （P 〈0.05） and began to decrease at the 14th day.The expressions of IGF-1 in treatment group were significantly obvious than that of control group at every time point after injury and there was significant difference between the two group （P 〈0.05）.The expression of AQP-4 in control group began to increase after one day of injury,reached the highest level at the third day,began to decrease at the 7th day and closed to normal level at the 28th day.Except for the 28th day the positive expressions of AQP-4 in treatment group at the first day,3rd day,7th day and 14th day were significantly lower than that of control.Conclusions The expression of IGF-1 and AQP-4 in normal retinal tissue is positive and it increases after incomplete injury of the optic nerve.IGF-1 and AQP-4 participate the process of incomplete injury of rats＇ optic nerve.Progesterone can decrease the expression of AQP-4 and increase the expression of IGF-1 in the rat retinal tissue after the incomplete injury of optic nerve.Progesterone can promo

关 键 词：视神经 黄体酮 腹腔注射 视网膜神经节细胞 胰岛素样生长因子-1 水通道蛋白4 

分 类 号：R573[医药卫生—消化系统]