mTORC1信号通路在狼疮鼠体内的表达及意义  

The expression of mTORC1 pathway in murine model of systemic lupus erythematosus

作　　者：谢双德 魏姗姗 张华年 王桂松 彭学标[1] 赵珂[3] 李俊灏 赖宽[1] XIE Shuangde;WEI Shanshan;ZHANG Huanian;WANG Guisong;PENG Xuebiao;ZHAO Ke;LI Jun-hao;LAI Kuan(Department of Dermatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Dermatology,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China;Department of Hematology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学南方医院皮肤科,广州510515 [2]南方医科大学珠江医院皮肤科,广州510282 [3]南方医科大学南方医院血液科,广州510515

出　　处：《中国麻风皮肤病杂志》2018年第8期449-452,共4页China Journal of Leprosy and Skin Diseases

基　　金：广东省自然科学基金(编号:2014A030313350)

摘　　要：目的:检测mTORC1信号通路在系统性红斑狼疮鼠体内的表达,探讨其在系统性红斑狼疮发病中的作用。方法:研究对象为6只B6.MRL/lpr狼疮小鼠和6只C57正常小鼠。RT-PCR和Western blot检测肾脏组织Akt、mTOR、p70S6K、IL-17 mRNA和蛋白的表达。细胞内染色流式细胞术检测脾脏Th17淋巴细胞亚群比例。结果:狼疮鼠组Akt、mTOR、p70S6K、IL-17 mRNA、磷酸化蛋白表达水平高于对照组(P<0.05);狼疮鼠组的Th17细胞占CD4^+T细胞的比例高于正常对照组(P<0.05)。结论:mTORC1(Akt-mTOR-p70S6K)信号通路在SLE的发病中起着重要作用。Objective： To detect the level of mTORC1 pathway related factors in the murine model of systemic lupus erythematosus（ SLE） and to explore its effect in the development of SLE. Methods： There were 6 B6.MRL/lpr lupus mice in the experimental group and 6 C57 mice in the control group. The levels of Akt,mTOR,p70S6K,IL-17 mRNA and protein in kidney were detected by RT-PCR and western blot. The percentage of Th17 lymphocytes in CD4^＋T cells in spleen was detected by flow cytometry. Results： The mRNA and phosphoprotein of Akt,mT OR,p70S6K and IL-17 in the experimental group were higher than those in the control group. The percentage of Th17 lymphocytes in CD4^＋T cells in the experimental group was higher than that in the control group. Conclusion： The activation of mTORC1（ Akt-mTOR-p70S6K） pathway may play an important role in the development of SLE.

关 键 词：系统性红斑狼疮 MTOR P70S6K TH17 

分 类 号：R593.241[医药卫生—内科学]