GPR88基因启动子区甲基化改变与甲基苯丙胺所致精神障碍相关性  

GPR88 promoter methylation change is associated with methamphetamine induced psychosis

作　　者：罗涛 董慧茜[2] 张陈茜[2] 刘亮[3] 郝伟[2] LUO Tao;DONG Huixi;ZHANG Chenxi(Jiangxi Mental Health Center,Nanchang 330029,China)

机构地区：[1]江西省精神卫生中心,南昌330029 [2]中南大学湘雅二医院精神卫生研究所 [3]无锡市精神卫生中心

出　　处：《国际精神病学杂志》2018年第4期591-593,603,共4页Journal Of International Psychiatry

基　　金：江西省卫生计生委科技计划项目:GPR88基因启动子区甲基化在甲基苯丙胺所致精神障碍的作用及机制研究(编号:20187078);国家自然科学基金重点项目:药物依赖易感性的遗传脑影像和发病机制研究(编号:81130020)

摘　　要：目的甲基苯丙胺所致精神障碍的机制尚不明确,以往的研究显示GPR88基因与精神病性症状的产生相关。本研究旨在探索GPR88基因启动子区甲基化水平与甲基苯丙胺所致精神障碍的相关性。方法采用Methylight qPCR技术测定106名男性甲基苯丙胺所致精神障碍者、118名男性甲基苯丙胺依赖未出现精神病性症状的患者及109名男性健康对照者GPR88基因启动子区甲基化水平。结果甲基苯丙胺所致精神障碍组GPR88基因启动子区甲基化水平显著高于甲基苯丙胺依赖未出现精神病性症状组及正常对照组,F值为71.80,P<0.001;进一步分析显示GPR88基因启动子区甲基化水平与患者目前的年龄、开始使用甲基苯丙胺的年龄、使用甲基苯丙胺的总时间、合并使用其它精神活性物质无显著相关性,而与患者精神病性症状的产生存在显著相关,相关系数为0.52,P<0.001。结论 GPR88基因启动子区甲基化水平与甲基苯丙胺所致精神障碍存在显著相关。Objective The mechanism of Methamphetamine induced psychosis（MAP）is not fully clear. The GPR88 gene encodes GPR88,an orphan G protein-coupled receptor enriched in the striatum and previously linked to behavioral traits relevant to psychotic symptoms. The aim of this study was to investigate the association of GPR88 gene promoter methylation with methamphetamine induced psychosis. Methods There were 106 male patients of methamphetamine induced psychosis,118 male patients dependent on methamphetamine without psychosis,and 109 agematched male controls recruited in this study. The methylight q PCR was performed to determine GPR88 gene promoter region methylation status. Results The methylation level of GPR88 gene promoter region is significantly increased in patients of methamphetamine induced psychosis than patients dependent on methamphetamine without psychosis and health control. Conclusions We report for the first time the significant association of GPR88 gene promoter methylation with methamphetamine induced psychosis.

关 键 词：甲基苯丙胺所致精神障碍 DNA甲基化 表观遗传 GPR88基因 

分 类 号：R749[医药卫生—神经病学与精神病学]