miRNA调控SCN2A参与癫痫的分子机制研究  

miRNA regulates epilepsy by targeting SCN2A

作　　者：文芳[1] 胡治平[1] 向军[1] 张凌云[1] 周玉[1] WEN Fang;HU Zhiping;XIANG Jun(Department of Neurology The Second Xiangya Hospital,Central South University,Changsha 410011,China)

机构地区：[1]中南大学湘雅二医院,长沙410011

出　　处：《国际精神病学杂志》2018年第4期693-696,共4页Journal Of International Psychiatry

基　　金：国家留学基金(编号:20147680)

摘　　要：目的在体内和体外癫痫模型中,探讨miRNA-132在癫痫中的作用及其机制。方法利用氯化锂-匹罗卡品构建大鼠颞叶癫痫模型及对照,通过real-time PCR检测miRNA的表达;利用Retinoic acid(RA)构建体外癫痫细胞模型,分别用Real-time PCR和Western blot检测miR-132-3p及SCN2A的miRNA及蛋白表达水平;在体外癫痫细胞模型中加入miR-132-3p的抑制剂,检测miR-132-3p及SCN2A的表达水平;在体内大鼠癫痫模型中,分析二者表达的相关性。结果在体外细胞癫痫模型中,miR-132-3p的miRNA和蛋白表达显著增加,而SCN2A的miRNA和蛋白表达显著下降;在体外细胞模型中证实miR-132-3p与SCN2A之间存在靶向抑制关系;在大鼠癫痫模型中证明miR-132-3p与SCN2A的表达呈现负相关关系。结论在体内和体外癫痫模型中,miR-132的表达升高,而其靶基因SCN2A的表达降低,且两者呈现负相关关系,表明miR-132通过其靶基因SCN2A参与癫痫的发生发展。Objective To investigate the role of miRNA-132 in in vivo and in vitro epilepsy models. MethodsConstructing rat model of temporal lobe epilepsy and control by using lithium and pilocarpine,the expression of miRNA was detected by real-time;Retinoic acid（RA）was used to construct epilepsy cell model in vitro;the miRNA and protein expression level of miR-132-3p and SCN2A were measured by Real-time PCR and Western blot;the miRNA and protein expression of SCN2A was measured in miR-132-3p inhititor treated cells;analysis of the correlation between the expression of miR-132-3p and SCN2A in vivo. Results The miRNA and protein expression of miR-132-3p was significantly increased,while the miRNA and protein expression of SCN2A was decreased significantly in in vitro model of epilepsy;miR-132-3p inhititor induced the expression of SCN2A in in vitro cell model of epilepsy;the expression of miR-132-3p was negatively related to SCN2A. Conclusion The expression of miR-132 was increased and the expression of its target gene SCN2A was decreased in in vivo and in vitro epilepsy models. The expression of miR-132-3p was negatively related to SCN2A,suggesting that miR-132 taken part in the development of epilepsy through its target gene SCN2A.

关 键 词：癫痫 大鼠模型 MIRNA SCN2A 

分 类 号：R749.99[医药卫生—神经病学与精神病学]