Construction of a high-efficiency gene-targeting system in brewing-wine Aspergillus oryzae industrial strain used in direct xylitol conversion from xylan  

作　　者：Hongwen Chen Yu Du Hongwei Guo 

机构地区：[1]College of Chemical Engineering, Huaqiao University, Xiamen 361021, China

出　　处：《Acta Biochimica et Biophysica Sinica》2018年第7期723-726,共4页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （No. 21476093）, the Natural Science Foundation of Fujian Province （No. 2018J01010）, the Fundamental Research Funds for the Central Universities （No. JB-ZR1112）, and the Scientific Research Fund of Huaqiao University （No. 12BS132）.

摘　　要：Aspergillus oryzae is an important industrial filamentous fungus widely applied in food processing and fermentation industry, and has been safely used for more than 1000 years. For an industrial brewing-wine strain A. oryzae, besides amylase and glucoamylase, it also secretes large amounts of xylanolytic enzymes, such as xylanase （EC 3.2.1.8）, f3-xylosidase （EC 3.2.1.37）, and c^-L-arabinofuranosi- dase （L-AF; EC 3.2.1.55）, to degrade xylan （in hemicellulose） into D-xylose [1]. In addition, it contains intracellular xylose-utilizing enzymes such as xylose reductase （XR; EC 1.1.1.21） and xylitol dehydrogenase （XDHA; EC 1.1.1.9） to transform D-xylose into use- ful xylitol [1]. Therefore, A.Aspergillus oryzae is an important industrial filamentous fungus widely applied in food processing and fermentation industry, and has been safely used for more than 1000 years. For an industrial brewing-wine strain A. oryzae, besides amylase and glucoamylase, it also secretes large amounts of xylanolytic enzymes, such as xylanase （EC 3.2.1.8）, f3-xylosidase （EC 3.2.1.37）, and c^-L-arabinofuranosi- dase （L-AF; EC 3.2.1.55）, to degrade xylan （in hemicellulose） into D-xylose [1]. In addition, it contains intracellular xylose-utilizing enzymes such as xylose reductase （XR; EC 1.1.1.21） and xylitol dehydrogenase （XDHA; EC 1.1.1.9） to transform D-xylose into use- ful xylitol [1]. Therefore, A.

分 类 号：Q933[生物学—微生物学] TQ920.1[轻工技术与工程—发酵工程]