可溶性PD-1/PD-L1在食管鳞癌癌变过程中的表达及意义  

Expression of soluble PD-1/PD-L1 in carcinogenesis of esophageal squamous carcinoma and its significance

作　　者：朱曼[1] 周福有[2] 原翔 郭晓锋 杨海军[4] ZHU Man;ZHOU Fuyou;YUAN Xiang;GUO Xiaofeng;YANG Haijun(College of Clinical Medicine,Henan University of Science and Technology,Luoyang,Henan 471003,China;Thoracic Surgery Department,Anyang Tumor Hospital,Anyang,Henan 455000,China;Laboratory,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang,Henan 471003,China;Pathology Department,Anyang Tumor Hospital,Anyang,Henan 455000,China)

机构地区：[1]河南科技大学临床医学院,河南洛阳471003 [2]河南省安阳市肿瘤医院胸外科,河南安阳455000 [3]河南科技大学第一附属医院实验室,河南洛阳471003 [4]河南省安阳市肿瘤医院病理科,河南安阳455000

出　　处：《中国医学工程》2018年第7期1-5,共5页China Medical Engineering

基　　金：国家博士后第69批基金项目(2016M602263)

摘　　要：目的检测人类可溶性细胞程序性死亡受体-1/人类可溶性程序性死亡配体-1(s PD-1/s PD-L1)在外周血中表达情况,分析其意义,为食管鳞癌的诊断和治疗提供理论基础。方法选取正常对照组、食管鳞癌前病变组、食管鳞癌组3组,每组90例。应用酶联免疫吸附试验(ELISA)检测待测人群血清中s PD-1/s PD-L1的含量,统计学分析其与临床病理学特征的关系。结果(1)s PD-1/s PD-L1在正常对照、食管鳞癌癌前病变、食管鳞癌三组外周血中的含量分别为(601.66±92.52)/(173.95±22.30)pg/ml、(636.62±125.79)/(195.49±28.44)pg/ml、(649.22±101.72)/(219.05±27.98)pg/m l,比较差异有统计学意义(P<0.05)。(2)3组外周血s PD-1和s PD-L1相关性分析,均无相关性。(3)食管鳞癌患者外周血s PD-1的表达与食管鳞癌N分期密切相关(P<0.05),s PD-L1与肿瘤的最大径密切相关(P<0.05)。(4)s PD-L1正常组和高值组的PFS有统计学差异(P=0.026)。结论 s PD-1与食管鳞癌病变的发生有关,s PD-L1与食管鳞癌的发生、发展及预后有一定的关系。【Objective】To discuss the expression of human s PD-1/s PD-L1 in peripheral blood and its significance, and provide theoretical basis for the diagnosis and treatment of esophageal squamous carcinoma.【Methods】The research objects were divided into the normal group, esophageal precancerous lesion group and esophageal squamous carcinoma group, with 90 cases in each group. The contents of s PD-1/s PD-L1 were detected by ezyme-linked immunosorbent assay（ELISA）. SPSS was used for statistical analysis.【Results】The contents of s PD-1/s PD-L1 in peripheral blood of the normal group, esophageal precancerous lesion group and esophageal squamous carcinoma group were respectively（601.66 ± 92.52）/（173.95 ± 22.30） pg/ml,（636.62 ± 125.79）/（195.49 ± 28.44） pg/ml,（649.22 ± 101.72）/（219.05 ± 27.98） pg/ml, and the differences were statistically significant（P〈0.05）. No significant association was observed between s PD-1 and s PD-L1 expression in the normal group, esophageal precancerous lesion group and esophageal squamous carcinoma group. The expression of s PD-1 in peripheral blood of patients with esophageal squamous carcinoma was closely correlated to tumor N status（P〈0.05）, and the expression of s PD-L1 was closely correlated to the maximum diameter of the tumor（P〈0.05）. Progression-free survival（PFS） was significantly poorer in the s PD-L1 high value group than in the s PD-L1 normal group（P =0.026）.【Conclusions】The s PD-1 is related to the occurrence of esophageal diseases, and the s PD-L1 is related to the genesis, progression and prognosis of esophageal squamous carcinoma.

关 键 词：食管鳞癌 SPD-1 sPD-L1 预后 

分 类 号：R735.1[医药卫生—肿瘤]