生物酶破胶剂产生菌的物理诱变及酶学性质研究  

Study on the physical mutagenesis and enzymatic properties of bio-enzyme breaking agent producing strain

作　　者：李慧玲[1] 程玉鹏[1] 刘祖艳[2] 赵敏[2] LI Hui-ling;CHENG Yu-peng;LIU Zu-yan;ZHAO Min(College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of Life Sciences,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《化学工程师》2018年第8期14-17,共4页Chemical Engineer

基　　金：黑龙江省自然科学基金项目(H2015042)

摘　　要：为了提高生物酶破胶剂β-甘露聚糖酶的活性,以产β-甘露聚糖酶的Bacillus subtilis W为出发菌株,经紫外线照射35～40s,酶活提高了8.63%;再经60Co诱变酶活提高了11.3%。粗酶液的最适p H值是6.2,最适温度是60℃,盐浓度在10%以下有促进酶促反应的作用。Bacillus subtilis W producing β-mannanase was used as starting strain to increase the β-mannanase activity of biological enzyme breaker. After 35-40 s UV irradiation, the enzyme activity increased by 8.63%60 Co mutagenesis activity increased by 11.3%. The optimum p H of the crude enzyme solution is 6.2, the optimum temperature is 60 ℃, the salt concentration below 10% to promote the enzymatic reaction.

关 键 词：生物酶破胶剂 物理诱变 酶学性质 

分 类 号：Q939.97[生物学—微生物学]