番茄水通道蛋白基因家族的生物信息学分析  

Bioinformatic Analysis of Tomato Aquaporin Gene Family

作　　者：李菲[1] 何小红[1] 李育柯 乙引[1] Li Fei;He Xiaohong;Li Yuke;Yi Yin(Key Laboratory of Plant Physiology and Development Regulation of Guizhou Province,School of Life Science,Guizhou Normal University,Guiyang,55002)

机构地区：[1]贵州师范大学生命科学学院植物生理与发育调控贵州省重点实验室

出　　处：《基因组学与应用生物学》2018年第9期3950-3957,共8页Genomics and Applied Biology

基　　金：贵州省科学技术基金(2017GZ66249)资助

摘　　要：植物水通道蛋白不仅仅在根系输送水分,而是具有底物和细胞定位的多样性的基因家族,因而对植物生理和发育过程,包括种子发芽、侧根发生、碳固定和营养吸收等都有重要的贡献。番茄基因组中共有44个水通道蛋白基因。这些基因可分为TIP、PIP、NIP和SIP 4个亚家族。这4个亚家族具有不同的功能和亚细胞定位。在番茄十号染色体水通道蛋白聚集位点上的5个基因与一号染色体上的一个基因是直系同源水通道蛋白,相似度都很高,同属于PIP亚家族。番茄水通道蛋白大多在根部表达量较高,在茎、叶、花等部位表达较低。不过Solyc03g096290.2.1和Solyc01g094690.2.1基因在花中表达量最高。此外,有个别基因表达量稳定在较高的水平,包括Solyc06g074820.2.1和Solyc08g081190.2.1。这些基因的功能可能不仅是吸收和转运水分,是否有其他的转运底物,在植物抗逆和发育过程中是否有调控作用,是下一步研究的重点。番茄全基因组中的水通道蛋白进行检索和分析,对番茄分子生物学研究和种质改良都有重要的意义。Plant aquaporins not only transport water in the root system, but also have a diversity of substrates and cell positioning, and thus are important for the plant physiology and development processes, including seed germination, lateral root occurrence, carbon fixation and nutrient absorption. There were 44 aquaporin genes in the tomato genome. These genes can be divided into TIP, PIP, NIP and SIP subfamilies. These four subfamilies have different functions and subcellular localization. The five aquaporin genes on tomato chromosome 10 and one aquaporin gene on chromosome 1 were orthologous aquaporins, and all of them belonged to PIP subfamily. The expression of aquaporin genes in tomato roots was higher than that in leaves and flowers. However,Solyc03 g096290.2.1 and Solyc01 g094690.2.1 had the highest expression in flowers. In addition, Solyc06 g074820.2.1 and Solyc08 g081190.2.1 expression levels were stable at high levels. These genes may function not only in the uptake and transportation of water, but also in the presence of other transport substrates, and further study is needed to identify whether they have a regulatory role in stress tolerance and development of plants. Tomato genome aquaporin genes search and analysis are of great significance for tomato molecular biology research and germplasm improvement.

关 键 词：番茄 水孔蛋白 系统进化 特异表达 

分 类 号：S641.2[农业科学—蔬菜学]