Ppgst基因的转录水平对纳米TiO_2的指示作用研究  

A Study on Transcriptional Response of a Glutathione S-transferase Gene of Physarum polycephalum to Nano-sized TiO_2

作　　者：张智 张天任[1] 张建华 刘士德 ZHANG Zhi;ZHANG Tianren;ZHANG Jianhua;LIU Shide(Shenzhen Key Laboratory of Microbial Genetic Engineering and Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science,College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518060,China;Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]深圳大学生命与海洋科学学院深圳市微生物基因工程重点实验室,广东深圳518060 [2]深圳大学光电工程学院光子器件与系统(教育部/广东省)重点实验室,广东深圳518060

出　　处：《环境科学与技术》2018年第8期66-70,共5页Environmental Science & Technology

基　　金：深圳市科技研发资金基础研究计划(JCYJ20170302144210545;JCYJ201418193546107;JC201105130450A);中国博士后科学基金(2017M622755);国家自然科学基金(31070043)

摘　　要：纳米二氧化钛Ti O2(nTiO_2)的大量使用与排放可能导致环境污染。因此有必要发展nTiO_2的环境污染标志物。该研究用多种粒径(25、43、67 nm)nTiO_2胁迫环境敏感型真菌多头绒泡菌,发现其谷胱甘肽酶(GST)总活性升高了8～44倍,活性高低与nTiO_2的胁迫浓度(5～15 mg/mL)呈现正相关;随后用PCR技术从被胁迫的该菌中克隆到1个谷胱甘肽转移酶(Ppgst)基因,进一步研究发现该基因会对nTiO_2的胁迫产生显著的转录应答,其转录水平与nTiO_2的胁迫浓度(5～15 mg/mL)呈正相关;该研究提示Ppgst基因可以作为生物标志物指示多种粒径nTiO_2的环境污染,具有潜在的应用价值。The widespread use of nano-sized Ti O2（nTiO2） and releasing into the environment are very likely to cause pollution so there is an urgent need to develop a biomarker for nTiO2 exposure. In this paper, the stress of nTiO2 with various diameters（25, 43, 67 nm） on an environmentally sensitive eukaryotic slime molds Physarum polycephalum was studied. It was found that the total enzyme activity of GST was increased by 8～44 fold with a positive correlation with the stress concentration of nTiO2（ranged 5～15 mg/mL）; then total RNA of P. polycephalum was isolated from the stressed P. polycephalum. The further findings were the transcriptional responses to the stress of nTiO2 of glutathione s-transferase gene（Ppgst）, and there existed a positive correlation between the transcriptional level and nTiO2 exposure concentrations within a range（5～15 mg/mL）. In conclusion, Ppgst could be used as a kind of biomarker to indicate the environmental pollution of nTiO2 of different diameters.

关 键 词：谷胱甘肽转移酶 纳米二氧化钛 多头绒泡菌 生物标志物 

分 类 号：X172[环境科学与工程—环境科学]