鲈鱼核型、Ag-NORs和C-带分析  

The Karyotype, Ag-NORs and C-Banding of Lateolabrax japonicus

作　　者：王晓艳 王世锋[2] 张建设 陈治 吴常文 高天翔 WANG Xiao-yan;WANG Shi-feng;ZHANG Jian-she(School of Ocean Science and Technology of Zhejiang Ocean University,National Engineering Research Center for Marine Aquaculture,Zhoushan 316022;Key Laboratory of Tropical Aquatic Biotechnology of Education Ministry,Hainan University,Haikou 570228,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院国家海洋设施养殖工程技术研究中心,浙江舟山316022 [2]海南大学热带生物资源可持续利用省部共建国家重点实验室培育基地,海南海口570228 [3]中国海洋大学水产学院渔业生态学实验室,山东青岛266003

出　　处：《浙江海洋大学学报（自然科学版）》2018年第4期285-291,共7页Journal of Zhejiang Ocean University：Natural Science

基　　金：浙江海洋大学高层次人才引进项目;海洋科学浙江省重中之重学科开放课题(20160202)

摘　　要：采用常规核型分析、Ag-NORs及C-带技术对鲈鱼的核型特征进行了研究。研究结果如下:(1)鲈鱼二倍体染色体数目为48(2n=48),全部为端着丝粒染色体(t型),染色体臂数FN=48,没有异形性染色体;(2)在所观察的中期分裂相中,第12对同源染色体次缢痕处显示为稳定的Ag-NORs,此处为C-带阴性;(3)鲈鱼染色体着丝粒处具有微弱C-带,第1对染色体靠近着丝粒位置为C-带强阳性;(4)在某些中期分类相中具有多银染位点。The karyotypic characteristics of Lateolabrax japonicus was investigated using conventional Giemsa-staining, Ag-NORs and C-banding methods. Results are as follows： （1） L. japonicus had a diploid chro- mosome number of 48, and all the karyotypes were formed by telocentric chromosomes （FN=48）. No heteromor- phic chromosome was detected; （2） the most stable Ag-NORs were interstitially located on the pericentromeric position of the 12th chromosome pair, and were coincident with the secondary constriction which appeared nega- tive C-banded; （3） faint C-bandings were observed at the centromeres, however, the conspicuous band were seen at the biggest chromosome pair; （4） multiple number of Ag-staining chromosomes were found.

关 键 词：鲈鱼 核型 AG-NORS C-带 

分 类 号：Q23[生物学—细胞生物学]