门静脉血Angiopoietin-2 mRNA定量检测与结直肠癌异时性肝转移及预后的关系  

Correlation between quantitative expression of Angiopoietin-2 mRNA in peripheral blood and metachronous liver metastasis and prognosis of patients with colorectal cancer

作　　者：刘少平[1] 胡亚华[1] 张海 何仁胜[1] 张惠芳[1] LIU Shaoping;HU Yahua;ZHANG Hai;HE Rensheng;ZHANG Huifang(Department of Gastroenterology,Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China)

机构地区：[1]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)消化内科,湖北黄石435000

出　　处：《胃肠病学和肝病学杂志》2018年第9期1049-1053,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：湖北省自然科学基金(2010CDB07401)

摘　　要：目的探讨结直肠癌(colorectal cancer,CRC)患者外周血和门静脉血中血管生成素-2 (Angiopoietin-2,Ang-2) mRNA的表达量与其肝转移及预后的关系。方法应用实时荧光定量RT-PCR检测89例CRC患者术前外周血和术中门静脉血Ang-2 mRNA表达量。所有患者术后随访3年。分析术中门静脉血Ang-2 mRNA表达量与CRC患者异时性肝转移、临床病理特征及预后的关系。结果异时性肝转移患者术前外周血和术中门静脉血Ang-2 mRNA表达量分别显著高于无异时性肝转移患者(P均<0. 01)。CRC患者术中门静脉血Ang-2 mRNA表达量与肿瘤分化程度、肿瘤大小、肿瘤浸润深度、是否有淋巴结转移、是否有术后复发、术后生存期长短密切相关(P <0. 05)。单因素分析显示,术前外周血和术中门静脉血Ang-2 mRNA表达量均与CRC异时性肝转移相关(χ2=4. 082,7. 389;P <0. 05);Cox多因素回归分析显示,两者均是其独立危险因素(相对危险度分别为4. 131,9. 625)。术中门静脉血Ang-2 mRNA高表达组患者的术后2年和3年生存率分别显著低于低表达组(P均<0. 05)。结论术中门静脉血Ang-2mRNA的表达量与CRC患者的重要临床病理特征及异时性肝转移、预后密切相关,其对异时性肝转移的预测价值比外周血Ang-2mRNA高,可成为CRC术后预后的评价指标。Objective To investigate the conelation between quantitative expression of Angiopoietin-2 （Ang-2） mRNA in portal vein blood and metachronous liver metastasis and prognosis of colorectal cancer （CRC） patients. Methods The quantitative expression of Ang-2 mRNA in intraoperative portal vein blood and preoperative peripheral blood were detec- ted by real-time fluorescence quantitative RT-PCR in 89 patients with CRC. The postoperative 3 years prognosis were tbllowed-up. The relationship between the intraoperative portal vein blood Ang-2 mRNA expression levels and the meta- chronous liver metastasis and prognosis of CRC were analyzed. Results The Ang-2 mRNA expression levels in preoper- ative peripheral blood and intraoperative portal vein blood in postoperative metachronous liver metastases group were sig- nificantly higher than those in the postoperative no metachronous liver metastases group （P 〈 0.01 ）. The Ang-2 mRNA expression levels in intraoperative portal vein blood of CRC group were positively related with the differentiated degree of tumor, tumor size, depth of invasion, lymph node metastasis, postoperative recurrence, postoperative survival （P 〈 0.05）. Univariate analysis revealed that the Ang-2 mRNA expression levels in intraoperative portal vein blood and pre- operative peripheral blood were all correlated to postoperative metachronous liver metastases from CRC 0（2 =4. 082, 7. 389; P 〈 0.05）. Multivariate Cox analysis showed that they were independent risk factors tbr postoperative metachronous liver metastases from CRC （relative risk values were 4. 131, 9. 625）. Two and three years of survival rate of the patients with high expression levels of Ang-2 mRNA in in intraoperative portal vein blood were significantly lower than those of the patients with low expression levels of Ang-2 mRNA （all P 〈 0.05）. Conclusion The Ang-2 mRNA expression levels in intraoperative portal vein blood are relatied with the important clinical and pathologic teature, prognosis and metachronous

关 键 词：结直肠癌 门静脉血 ANG-2 mRNA 异时性肝转移 预后 

分 类 号：R735.3[医药卫生—肿瘤]