PTEN-Long通过PI3K-AKT途径对HepG2肝癌细胞的影响  

Effects of PTEN-Long on PI3K-AKT Pathway in HepG2 Cells

作　　者：张谢[1] 谭麟[2] 毛琦淇 李宏[1] ZHANG Xie;TAN Lin;MAYO Qi-qi;LI Hong(Ningbo Medical Treatment Center Li Huili Hospital,Ningbo 315040,China;School of Medicine,Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波市医疗中心李惠利医院,浙江宁波315040 [2]宁波大学医学院,浙江宁波315211

出　　处：《肿瘤学杂志》2018年第8期764-768,共5页Journal of Chinese Oncology

基　　金：浙江省公益技术应用研究计划项目(2017C35002);宁波市重大民生项目(2013C51009);浙江省医药卫生平台计划项目(2016DTA009)

摘　　要：[目的]探讨PTEN-Long对肝细胞癌HepG2细胞的影响及其脂质磷酸酶活性与PI3K-AKT信号通路之间的关系。[方法]细胞转染法转染PTEN-Long、PTEN-LongG302R和Vector于HepG2细胞。转染48h后,检测各组细胞增殖数,Westernblot法检测各组细胞PTEN-Long、pAkt、pS6K表达变化,流式细胞法检测各组细胞细胞凋亡水平变化,细胞划痕法检测各组细胞迁移情况。[结果]细胞转染后,PTEN-Long组、PTEN-LongG320R组表达PTENLong蛋白量无显著性差异;与Vector、PTEN-LongG320R组相比,PTEN-Long组pAkt（Ser473）、pS6K（Thr389）表达量明显下降。从转染后48h开始,PTEN-Long组细胞增殖数较Vector组、PTEN-LongG320R组明显减少,差异有统计学意义（P〈0.05）;PTEN-Long组较Vector组、PPTENLongG320R组凋亡明显增强（P〈0.05）;划痕14h后,PTEN-Long组、PTEN-LongG320R组较Vector组的迁移细胞数明显减少（P〈0.05）。[结论]PTEN-long依赖于其脂质磷酸酶活性,通过PI3KAKT通路调控肝癌HepG2细胞的增殖与凋亡。[Objective] To investigate the effect of PTEN-Long on hepatocellular carcinoma HepG2 cells and the relationship between lipid phosphatase activity and PI3 K-AKT signaling pathway. [Methods ] PTEN-Long,PTEN-LongG302 Rand Vector were transfected to HepG2 cells,respectively. After transfection for 48 h,cell proliferation was detected in three groups,the expression of PTEN-Long,p Akt and p S6 K was detected by Western blot,cell apoptosis was detected by flow cytometry,cell migration was detected by cell scratch assay. [Results] After transfection for48 h,there was no significant difference in expression of PTEN-Long protein between PTEN-Long group and PTEN-LongG320 Rgroup. Compared with Vector and PTEN-LongG320 Rgroup,the expression of p Akt（Ser473） and pS6 K（Thr389） in PTEN-Long group decreased significantly. From 48 h after transfection,the cell proliferation in PTEN-Long group was significantly lower than that of PTENLongG320 Rgroup and Vector group（P〈0.05）. The apoptosis rate in PTEN-Long group was significantly higher than that in vector group and PPTEN-LongG320 Rgroup（P〈0.05）. The scratch assay showed that the number of migrating cells in PTEN-Long group and PTEN-LongG320 Rgroup was significantly lower than that in Vector group（P〈0.05）. [Conclusion] PTEN-long regulates the proliferation and apoptosis of hepatocellular carcinoma Hep G2 cells through PI3 K-AKT pathway,which is lipid phosphatase activity-dependent.

关 键 词：磷酸酶及张力蛋白同源基因剪接体 HEPG2细胞系 细胞凋亡 磷酸肌醇3-激酶/蛋白激酶B 

分 类 号：R735.7[医药卫生—肿瘤]